• Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using EIF1AY Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-EIF1AY antibody (21-70 aa) (STJ92868)

SKU:
STJ92868

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Eukaryotic translation initiation factor 1A, Y-chromosomal (21-70 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EIF1AY
Gene ID: 9086
Uniprot ID: IF1AY_HUMAN
Immunogen Region: 21-70 aa
Specificity: eIF1AY Polyclonal Antibody detects endogenous levels of eIF1AY protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human EIF1AY at the amino acid range 21-70
Function Component of the 43S pre-initiation complex (43S PIC), which binds to the mRNA cap-proximal region, scans mRNA 5'-untranslated region, and locates the initiation codon. This protein enhances formation of the cap-proximal complex. Together with EIF1, facilitates scanning, start codon recognition, promotion of the assembly of 48S complex at the initiation codon (43S PIC becomes 48S PIC after the start codon is reached), and dissociation of aberrant complexes. After start codon location, together with EIF5B orients the initiator methionine-tRNA in a conformation that allows 60S ribosomal subunit joining to form the 80S initiation complex. Is released after 80S initiation complex formation, just after GTP hydrolysis by EIF5B, and before release of EIF5B. Its globular part is located in the A site of the 40S ribosomal subunit. Its interaction with EIF5 during scanning contribute to the maintenance of EIF1 within the open 43S PIC. In contrast to yeast orthologs, does not bind EIF1.
Protein Name Eukaryotic Translation Initiation Factor 1a - Y-Chromosomal
Eif-1a Y Isoform
Eif1a Y Isoform
Eukaryotic Translation Initiation Factor 4c
Eif-4c
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Eukaryotic Translation Initiation Factor 1a - Y-Chromosomal antibody
Anti-Eif-1a Y Isoform antibody
Anti-Eif1a Y Isoform antibody
Anti-Eukaryotic Translation Initiation Factor 4c antibody
Anti-Eif-4c antibody
Anti-EIF1AY antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance