| Host: |
Rabbit |
| Applications: |
WB/IP/ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:1000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
EHMT2 |
| Gene ID: |
10919 |
| Uniprot ID: |
EHMT2_HUMAN |
| Immunogen Region: |
877-1176 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 877-1176 of human EHMT2 (NP_079532.5). |
| Immunogen Sequence: |
VGNRAIRTEKIICRDVARGY ENVPIPCVNGVDGEPCPEDY KYISENCETSTMNIDRNITH LQHCTCVDDCSSSNCLCGQL SIRCWYDKDGRLLQEFNKIE PPLIFECNQACSCWRNCKNR VVQSGIKVRLQLYRTAKMGW GVRALQTIPQGTFICEYVGE LISDAEADVREDDSYLFDLD NKDGEVYCIDARYYGNISRF INHLCDPNIIPVRVFMLHQD LRFPRIAFFSSRDIRTGEE |
| Tissue Specificity | Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow. |
| Post Translational Modifications | Methylated at Lys-185.automethylated. |
| Function | Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself. |
| Protein Name | Histone-Lysine N-Methyltransferase Ehmt2Euchromatic Histone-Lysine N-Methyltransferase 2Hla-B-Associated Transcript 8Histone H3-K9 Methyltransferase 3H3-K9-Hmtase 3Lysine N-Methyltransferase 1cProtein G9a |
| Database Links | Reactome: R-HSA-2559582Reactome: R-HSA-3214841Reactome: R-HSA-427389Reactome: R-HSA-6804760Reactome: R-HSA-73762Reactome: R-HSA-8853884Reactome: R-HSA-8953750Reactome: R-HSA-9843970 |
| Cellular Localisation | NucleusChromosomeAssociates With Euchromatic RegionsDoes Not Associate With Heterochromatin |
| Alternative Antibody Names | Anti-Histone-Lysine N-Methyltransferase Ehmt2 antibodyAnti-Euchromatic Histone-Lysine N-Methyltransferase 2 antibodyAnti-Hla-B-Associated Transcript 8 antibodyAnti-Histone H3-K9 Methyltransferase 3 antibodyAnti-H3-K9-Hmtase 3 antibodyAnti-Lysine N-Methyltransferase 1c antibodyAnti-Protein G9a antibodyAnti-EHMT2 antibodyAnti-BAT8 antibodyAnti-C6orf30 antibodyAnti-G9A antibodyAnti-KMT1C antibodyAnti-NG36 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
