Anti-EHMT1 antibody [1B7B4] (STJ98015)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenPurified recombinant fragment of EHMT1 expressed in E. Coli.
General Information
| Short Description | Mouse monoclonal anti-Histone-lysine N-methyltransferase EHMT1 for use in WB and ELISA in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 1B7B4 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity purification |
| Dilution Range | WB 1:500-1:2000ELISA 1:10000 |
| Formulation | Liquid in PBS containing 0.03% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EHMT1 |
| Gene ID | 79813 |
| Uniprot ID | EHMT1_HUMAN |
| Immunogen | Purified recombinant fragment of EHMT1 expressed in E. Coli. |
| Specificity | EHMT1 Monoclonal Antibody detects endogenous levels of EHMT1 protein. |
Additional Info
| Function | Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Represses the expression of mitochondrial function-related genes, perhaps by occupying their promoter regions, working in concert with probable chromatin reader BAZ2B. |
| Protein Name | Histone-Lysine N-Methyltransferase Ehmt1Euchromatic Histone-Lysine N-Methyltransferase 1Eu-Hmtase1G9a-Like Protein 1GlpGlp1Histone H3-K9 Methyltransferase 5H3-K9-Hmtase 5Lysine N-Methyltransferase 1d |
| Database Links | Reactome: R-HSA-2559582Reactome: R-HSA-3214841Reactome: R-HSA-6804760Reactome: R-HSA-8853884Reactome: R-HSA-8953750Reactome: R-HSA-9843970 |
| Cellular Localisation | NucleusChromosomeAssociates With Euchromatic Regions |
| Alternative Antibody Names | Anti-Histone-Lysine N-Methyltransferase Ehmt1 antibodyAnti-Euchromatic Histone-Lysine N-Methyltransferase 1 antibodyAnti-Eu-Hmtase1 antibodyAnti-G9a-Like Protein 1 antibodyAnti-Glp antibodyAnti-Glp1 antibodyAnti-Histone H3-K9 Methyltransferase 5 antibodyAnti-H3-K9-Hmtase 5 antibodyAnti-Lysine N-Methyltransferase 1d antibodyAnti-EHMT1 antibodyAnti-EUHMTASE1 antibodyAnti-GLP antibodyAnti-KIAA1876 antibodyAnti-KMT1D antibody |
Information sourced from Uniprot.org