| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-EHMT1 (1-260) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
EHMT1 |
| Gene ID: |
79813 |
| Uniprot ID: |
EHMT1_HUMAN |
| Immunogen Region: |
1-260 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human EHMT1 (NP_079033.4). |
| Immunogen Sequence: |
MAAADAEAVPARGEPQQDCC VKTELLGEETPMAADEGSAE KQAGEAHMAADGETNGSCEN SDASSHANAAKHTQDSARVN PQDGTNTLTRIAENGVSERD SEAAKQNHVTADDFVQTSVI GSNGYILNKPALQAQPLRTT STLASSLPGHAAKTLPGGAG KGRTPSAFPQTPAAPPATLG EGSADTEDRKLPAPGADVKV HRARKTMPKSVVGLHAASKD PREVREARDHKEPKEEINK |
| Tissue Specificity | Widely expressed. |
| Function | Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Represses the expression of mitochondrial function-related genes, perhaps by occupying their promoter regions, working in concert with probable chromatin reader BAZ2B. |
| Protein Name | Histone-Lysine N-Methyltransferase Ehmt1Euchromatic Histone-Lysine N-Methyltransferase 1Eu-Hmtase1G9a-Like Protein 1GlpGlp1Histone H3-K9 Methyltransferase 5H3-K9-Hmtase 5Lysine N-Methyltransferase 1d |
| Database Links | Reactome: R-HSA-2559582Reactome: R-HSA-3214841Reactome: R-HSA-6804760Reactome: R-HSA-8853884Reactome: R-HSA-8953750 |
| Cellular Localisation | NucleusChromosomeAssociates With Euchromatic Regions |
| Alternative Antibody Names | Anti-Histone-Lysine N-Methyltransferase Ehmt1 antibodyAnti-Euchromatic Histone-Lysine N-Methyltransferase 1 antibodyAnti-Eu-Hmtase1 antibodyAnti-G9a-Like Protein 1 antibodyAnti-Glp antibodyAnti-Glp1 antibodyAnti-Histone H3-K9 Methyltransferase 5 antibodyAnti-H3-K9-Hmtase 5 antibodyAnti-Lysine N-Methyltransferase 1d antibodyAnti-EHMT1 antibodyAnti-EUHMTASE1 antibodyAnti-GLP antibodyAnti-KIAA1876 antibodyAnti-KMT1D antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89724/358205/STJ11100788_1__51624.1713122526.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
