• Western blot analysis of various lysates using Egr4 Rabbit polyclonal antibody (STJ118797) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Egr4 antibody (1-367) (STJ118797)

SKU:
STJ118797

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EGR4
Gene ID: 1961
Uniprot ID: EGR4_HUMAN
Immunogen Region: 1-367
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-367 of mouse Egr4 (NP_065621.1).
Immunogen Sequence: MLHLSDFSGSDALLSKPTEG CAHASPELPRLPARDAPSAA AYPGGDFLSWALSSCGAGGD LTDSCFLEGPAPTPPSGLSY SGSFFIQAVPEHPHDPEALF NLMSGILGLAPFPGPEAAAS RSPLDVPFPAGPDALLPDLY SPDLSSAAFPEAFWEAAPSA GAPSQCLFEPQLSPPDVKPG LRAPPASPALDAAASAFKGP YAPWELLSAGVPGNCGSQGS FQTTPEARFSAVGTKVEDL
Function Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-GCGGGGGCG-3' (GSG). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.
Protein Name Early Growth Response Protein 4
Egr-4
At133
Database Links Reactome: R-HSA-9031628
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Early Growth Response Protein 4 antibody
Anti-Egr-4 antibody
Anti-At133 antibody
Anti-EGR4 antibody

Information sourced from Uniprot.org

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