• Western blot analysis of extracts of various cell lines, using EGLN1/EGLN2 antibody (STJ112380) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EGLN1/EGLN2 antibody (250-350) (STJ112380)

SKU:
STJ112380

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EGLN1
EGLN2
Gene ID: 54583
112398
Uniprot ID: EGLN1_HUMAN
EGLN2_HUMAN
Immunogen Region: 250-350
Specificity: A synthetic peptide corresponding to a sequence within amino acids 250-350 of human EGLN1/EGLN2 (NP_071334.1/NP_444274.1).
Immunogen Sequence: DIRGDKITWIEGKEPGCETI GLLMSSMDDLIRHCNGKLGS YKINGRTKAMVACYPGNGTG YVRHVDNPNGDGRCVTCIYY LNKDWDAKVSGGILRIFPEG K
Tissue Specificity Q9GZT9 - widely expressed with highest levels in skeletal muscle and heart, moderate levels in pancreas, brain (dopaminergic neurons of adult and fetal substantia nigra) and kidney, and lower levels in lung and liver. widely expressed with highest levels in brain, kidney and adrenal gland. Expressed in cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle. .expressed in adult and fetal heart, brain, liver, lung, skeletal muscle and kidney. Also expressed in placenta. Highest levels in adult heart, brain, lung and liver and fetal brain, heart spleen and skeletal muscle.
Q96KS0 - Expressed in adult and fetal heart, brain, liver, lung, skeletal muscle, and kidney. Also expressed in testis and placenta. Highest levels in adult brain, placenta, lung, kidney, and testis. Expressed in hormone responsive tissues, including normal and cancerous mammary, ovarian and prostate epithelium.
Alternative Antibody Names Anti-Egl Nine Homolog 1 antibody
Anti-Hypoxia-Inducible Factor Prolyl Hydroxylase 2 antibody
Anti-Hif-Ph2 antibody
Anti-Hif-Prolyl Hydroxylase 2 antibody
Anti-Hph-2 antibody
Anti-Prolyl Hydroxylase Domain-Containing Protein 2 antibody
Anti-Phd2 antibody
Anti-Sm-20 antibody
Anti-EGLN1 antibody
Anti-C1orf12 antibody
Anti-PNAS-118 antibody
Anti-PNAS-137 antibody
Anti-Prolyl Hydroxylase Egln2 antibody
Anti-Egl Nine Homolog 2 antibody
Anti-Estrogen-Induced Tag 6 antibody
Anti-Eit-6 antibody
Anti-Hph-3 antibody
Anti-Hypoxia-Inducible Factor Prolyl Hydroxylase 1 antibody
Anti-Hif-Ph1 antibody
Anti-Hif-Prolyl Hydroxylase 1 antibody
Anti-Hph-1 antibody
Anti-Prolyl Hydroxylase Domain-Containing Protein 1 antibody
Anti-Phd1 antibody
Anti-EGLN2 antibody
Anti-EIT6 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance