| Host: | Rabbit |
| Applications: | WB/DB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 DB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | EGF |
| Gene ID: | 1950 |
| Uniprot ID: | EGF_HUMAN |
| Immunogen Region: | 971-1023 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 971-1023 of human EGF (NP_001954.2). |
| Immunogen Sequence: | NSDSECPLSHDGYCLHDGVC MYIEALDKYACNCVVGYIGE RCQYRDLKWWELR |
| Tissue Specificity | Expressed in kidney, salivary gland, cerebrum and prostate. |
| Post Translational Modifications | O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor). |
| Function | EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro. |
| Protein Name | Pro-Epidermal Growth Factor Egf Cleaved Into - Epidermal Growth Factor Urogastrone |
| Database Links | Reactome: R-HSA-114608 Reactome: R-HSA-1227986 Reactome: R-HSA-1236382 Reactome: R-HSA-1236394 Reactome: R-HSA-1250196 Reactome: R-HSA-1251932 Reactome: R-HSA-1257604 Reactome: R-HSA-177929 Reactome: R-HSA-179812 Reactome: R-HSA-180292 Reactome: R-HSA-180336 Reactome: R-HSA-182971 Reactome: R-HSA-1963640 Reactome: R-HSA-1963642 Reactome: R-HSA-212718 Reactome: R-HSA-2219530 Reactome: R-HSA-5637810 Reactome: R-HSA-5638303 Reactome: R-HSA-5673001 Reactome: R-HSA-6785631 Reactome: R-HSA-6811558 Reactome: R-HSA-8847993 Reactome: R-HSA-8856825 Reactome: R-HSA-8856828 Reactome: R-HSA-8863795 Reactome: R-HSA-9009391 Reactome: R-HSA-9013507 Reactome: R-HSA-9634638 Reactome: R-HSA-9664565 Reactome: R-HSA-9665348 Reactome: R-HSA-9665686 Reactome: R-HSA-9725554 Reactome: R-HSA-9818030 |
| Cellular Localisation | Membrane Single-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-Pro-Epidermal Growth Factor antibody Anti-Egf Cleaved Into - Epidermal Growth Factor antibody Anti-Urogastrone antibody Anti-EGF antibody |
Information sourced from Uniprot.org