| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | EEF2K |
| Gene ID: | 29904 |
| Uniprot ID: | EF2K_HUMAN |
| Immunogen Region: | 406-725 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 406-725 of human EEF2K (NP_037434.1). |
| Immunogen Sequence: | KLDHLHWPVFSDLDNMASRD HDHLDNHRESENSGDSGYPS EKRGELDDPEPREHGHSYSN RKYESDEDSLGSSGRVCVEK WNLLNSSRLHLPRASAVALE VQRLNALDLEKKIGKSILGK VHLAMVRYHEGGRFCEKGEE WDQESAVFHLEHAANLGELE AIVGLGLMYSQLPHHILADV SLKETEENKTKGFDYLLKAA EAGDRQSMILVARAFDSGQN LSPDRCQDWLEALHWYNTA |
| Post Translational Modifications | Autophosphorylated at multiple residues, Thr-348 being the major site. Phosphorylated by AMP-activated protein kinase AMPK at Ser-398 leading to EEF2K activation and protein synthesis inhibition. Phosphorylated by TRPM7 at Ser-78 resulting in improved protein stability, higher EE2F phosphorylated and subsequently reduced rate of protein synthesis. Phosphorylation by other kinases such as CDK1 and MAPK13 at Ser-359 or RPS6KA1 and RPS6KB1 at Ser-366 instead decrease EEF2K activity and promote protein synthesis. |
| Function | Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced. |
| Protein Name | Eukaryotic Elongation Factor 2 Kinase Eef-2 Kinase Eef-2k Calcium/Calmodulin-Dependent Eukaryotic Elongation Factor 2 Kinase |
| Database Links | Reactome: R-HSA-166208 |
| Alternative Antibody Names | Anti-Eukaryotic Elongation Factor 2 Kinase antibody Anti-Eef-2 Kinase antibody Anti-Eef-2k antibody Anti-Calcium/Calmodulin-Dependent Eukaryotic Elongation Factor 2 Kinase antibody Anti-EEF2K antibody |
Information sourced from Uniprot.org
