• Immunocytochemistry staining of HeLa cells fixed with-20℃ Methanol and using anti-eEF2 mouse mAb (dilution 1:200).
  • Western blot detection of eEF2 in HL-60, Jurkat, SHSY-5Y, U20S and Hela cell lysates using eEF2 mouse mAb (1:5000 diluted).Predicted band size:95KDa.Observed band size: 95KDa.Exposure time:15s.

Anti-EEF2 antibody [4B3-G7-H5] (STJ99117)

SKU:
STJ99117

£139.00 - £336.00
Current Stock:
Host: Mouse
Applications: WB/IHC/IF/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Elongation factor 2 is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Monoclonal
Clone ID: 4B3-G7-H5
Conjugation: Unconjugated
Isotype: IgG2b
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:5000
ICC 1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EEF2
Gene ID: 1938
Uniprot ID: EF2_HUMAN
Specificity: This antibody detects endogenous levels of eEF2 and does not cross-react with related proteins.
Immunogen: Purified recombinant human eEF2 protein fragments expressed in E.coli.
Function Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Protein Name Elongation Factor 2
Ef-2
Database Links Reactome: R-HSA-156902
Reactome: R-HSA-5336415
Reactome: R-HSA-5358493
Reactome: R-HSA-6798695
Reactome: R-HSA-8876725
Cellular Localisation Cytoplasm
Nucleus
Phosphorylation By Csk Promotes Cleavage And Sumoylation-Dependent Nuclear Translocation Of The C-Terminal Cleavage Product
Alternative Antibody Names Anti-Elongation Factor 2 antibody
Anti-Ef-2 antibody
Anti-EEF2 antibody
Anti-EF2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance