• Immunofluorescence analysis of L929 cells using EEF1E1 Rabbit polyclonal antibody (STJ117552) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using EEF1E1 Rabbit polyclonal antibody (STJ117552) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-EEF1E1 antibody (1-100) (STJ117552)

SKU:
STJ117552

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EEF1E1 (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EEF1E1
Gene ID: 9521
Uniprot ID: MCA3_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EEF1E1 (NP_004271.1).
Immunogen Sequence: MAAAAELSLLEKSLGLSKGN KYSAQGERQIPVLQTNNGPS LTGLTTIAAHLVKQANKEYL LGSTAEEKAIVQQWLEYRVT QVDGHSSKNDIHTLLKDLNS
Tissue Specificity Down-regulated in various cancer tissues.
Function Positive modulator of ATM response to DNA damage.
Protein Name Eukaryotic Translation Elongation Factor 1 Epsilon-1
Aminoacyl Trna Synthetase Complex-Interacting Multifunctional Protein 3
Elongation Factor P18
Multisynthase Complex Auxiliary Component P18
Database Links Reactome: R-HSA-2408522
Reactome: R-HSA-379716
Cellular Localisation Cytoplasm
Cytosol
Nucleus
Cytoplasmic Under Growth Arrest Conditions
Translocated Into The Nucleus When Growth Resumes (S Phase) And Following Dna Damage
Alternative Antibody Names Anti-Eukaryotic Translation Elongation Factor 1 Epsilon-1 antibody
Anti-Aminoacyl Trna Synthetase Complex-Interacting Multifunctional Protein 3 antibody
Anti-Elongation Factor P18 antibody
Anti-Multisynthase Complex Auxiliary Component P18 antibody
Anti-EEF1E1 antibody
Anti-AIMP3 antibody
Anti-P18 antibody

Information sourced from Uniprot.org

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