| Host: | Rabbit |
| Applications: | WB/IF/ICC/IP/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | EED |
| Gene ID: | 8726 |
| Uniprot ID: | EED_HUMAN |
| Immunogen Region: | 1-400 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-400 of human EED (NP_694536.1). |
| Immunogen Sequence: | MSEREVSTAPAGTDMPAAKK QKLSSDENSNPDLSGDENDD AVSIESGTNTERPDTPTNTP NAPGRKSWGKGKWKSKKCKY SFKCVNSLKEDHNQPLFGVQ FNWHSKEGDPLVFATVGSNR VTLYECHSQGEIRLLQSYVD ADADENFYTCAWTYDSNTSH PLLAVAGSRGIIRIINPITM QCIKHYVGHGNAINELKFHP RDPNLLLSVSKDHALRLWNI QTDTLVAIFGGVEGHRDEV |
| Tissue Specificity | Expressed in brain, colon, heart, kidney, liver, lung, muscle, ovary, peripheral blood leukocytes, pancreas, placenta, prostate, spleen, small intestine, testis, thymus and uterus. Appears to be overexpressed in breast and colon cancer. |
| Post Translational Modifications | Methylated. Binding to histone H1 'Lys-26' promotes mono-, di-, and trimethylation of internal lysines. |
| Function | Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. |
| Protein Name | Polycomb Protein EedHeedEmbryonic Ectoderm Development ProteinWd Protein Associating With Integrin Cytoplasmic Tails 1Wait-1 |
| Database Links | Reactome: R-HSA-212300Reactome: R-HSA-2559580Reactome: R-HSA-3214841Reactome: R-HSA-5617472Reactome: R-HSA-8943724Reactome: R-HSA-8953750Reactome: R-HSA-9609690Reactome: R-HSA-9710421 |
| Cellular Localisation | NucleusChromosomeTransiently Colocalizes With Xist At Inactive X Chromosomes |
| Alternative Antibody Names | Anti-Polycomb Protein Eed antibodyAnti-Heed antibodyAnti-Embryonic Ectoderm Development Protein antibodyAnti-Wd Protein Associating With Integrin Cytoplasmic Tails 1 antibodyAnti-Wait-1 antibodyAnti-EED antibody |
Information sourced from Uniprot.org