• Immunohistochemistry analysis of paraffin-embedded Mouse kidney using EEA1 antibody (STJ110997) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Human mammary cancer using EEA1 antibody (STJ110997) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Rat lung using EEA1 antibody (STJ110997) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using EEA1 antibody (STJ110997) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-EEA1 antibody (1182-1411) (STJ110997)

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STJ110997

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EEA1 (1182-1411) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EEA1
Gene ID: 8411
Uniprot ID: EEA1_HUMAN
Immunogen Region: 1182-1411
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1182-1411 of human EEA1 (NP_003557.2).
Immunogen Sequence: LKAAVEQEKRNQQILKDQVK KEEEELKKEFIEKEAKLHSE IKEKEVGMKKHEENEAKLTM QITALNENLGTVKKEWQSSQ RRVSELEKQTDDLRGEIAVL EATVQNNQDERRALLERCLK GEGEIEKLQTKVLELQRKLD NTTAAVQELGRENQSLQIKH TQALNRKWAEDNEVQNCMAC GKGFSVTVRRHHCRQCGNIF CAECSAKNALTPSSKKPVRV CDACFNDLQG
Function Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
Protein Name Early Endosome Antigen 1
Endosome-Associated Protein P162
Zinc Finger Fyve Domain-Containing Protein 2
Database Links Reactome: R-HSA-168138
Cellular Localisation Cytoplasm
Early Endosome Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Early Endosome Antigen 1 antibody
Anti-Endosome-Associated Protein P162 antibody
Anti-Zinc Finger Fyve Domain-Containing Protein 2 antibody
Anti-EEA1 antibody
Anti-ZFYVE2 antibody

Information sourced from Uniprot.org

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