| Host: | Rabbit |
| Applications: | ELISA/WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-EDG8 (380-400) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Purification: | Affinity Purified |
| Concentration: | 0.52-0.63 µg/µl |
| Dilution Range: | WB: 1:500 DB: 1:10, 000 ELISA: 1:10, 000 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | S1pr5 |
| Gene ID: | 60399 |
| Uniprot ID: | S1PR5_RAT |
| Immunogen Region: | 380-400 |
| Specificity: | Does not cross eact to other Spingosine 1-phosphate receptor subtypes. Labels a strong band of EDG8 of 48-50 kDa. Also labels a 54 kDa band, the identity of this is not known but may represent phosphorylated EDG8. |
| Immunogen: | Synthetic peptide taken within amino acid region 380-400 of rat S1P5R/EDG8 protein. |
| Tissue Specificity | Found almost exclusively in brain and skin. Ubiquitously detected in different brain regions with very prominent expression in lower brain regions such as the midbrain, pons, medulla, and spinal cord. Expressed predominantly within white matter tracts of the brain. Corpus callosum, optic nerve, olfactory tract, anterior commissure, internal and external capsules, fimbra of the hippocampus, mammillary tract, stria medullaris, and white matter of the cerebellum and brain stem all express the receptor. Within the striatum itself white matter fascicles express the receptor. Detected also in spleen and lung. Not observed in heart, liver, skeletal muscle, kidney, or testes. |
| Function | Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. Is coupled to both the G(i/O)alpha and G(12) subclass of heteromeric G-proteins. Displays antiproliferative effects in transfected CHO-K1 and HEK293 cells. |
| Protein Name | Sphingosine 1-Phosphate Receptor 5 S1p Receptor 5 S1p5 Endothelial Differentiation G-Protein-Coupled Receptor 8 Nerve Growth Factor-Regulated G-Protein-Coupled Receptor 1 Nrg-1 Sphingosine 1-Phosphate Receptor Edg-8 S1p Receptor Edg-8 |
| Database Links | Reactome: R-RNO-418594 Reactome: -RNO-419408 |
| Cellular Localisation | Cell Membrane Multi-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Sphingosine 1-Phosphate Receptor 5 antibody Anti-S1p Receptor 5 antibody Anti-S1p5 antibody Anti-Endothelial Differentiation G-Protein-Coupled Receptor 8 antibody Anti-Nerve Growth Factor-Regulated G-Protein-Coupled Receptor 1 antibody Anti-Nrg-1 antibody Anti-Sphingosine 1-Phosphate Receptor Edg-8 antibody Anti-S1p Receptor Edg-8 antibody Anti-S1pr5 antibody Anti-Edg8 antibody Anti-Nrg1 antibody |
Information sourced from Uniprot.org