| Short Description : | Rabbit polyclonal anti-Endothelial differentiation-related factor 1 for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | 1 mg/mL |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range: | WB 1:500-2000 |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | EDF1 |
| Gene ID: | 8721 |
| Uniprot ID: | EDF1_HUMAN |
| Immunogen: | Synthesized peptide derived from human EDF1 |
| Specificity: | This antibody detects endogenous levels of EDF1 at Human, Mouse, Rat |
| Post Translational Modifications | Phosphorylated (by PKA and PKC). |
| Function | Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. May function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism. |
| Protein Name | Endothelial Differentiation-Related Factor 1Edf-1Multiprotein-Bridging Factor 1Mbf1 |
| Database Links | |
| Cellular Localisation | CytoplasmNucleusAlso Nuclear Upon Binding To Nr5a1 And Treatment Of Cells With Tpa Or Forskolin |
| Alternative Antibody Names | Anti-Endothelial Differentiation-Related Factor 1 antibodyAnti-Edf-1 antibodyAnti-Multiprotein-Bridging Factor 1 antibodyAnti-Mbf1 antibodyAnti-EDF1 antibody |
Information sourced from Uniprot.org
![Immunohistochemistry analysis of EDF1 in paraffin-embedded mouse lung using [KO Validated] EDF1 Rabbit polyclonal antibody (STJ23470) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/99544/384668/STJ23470_1__14722.1713153436.jpg?c=1 "Immunohistochemistry analysis of EDF1 in paraffin-embedded mouse lung using [KO Validated] EDF1 Rabbit polyclonal antibody (STJ23470) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
![Western blot analysis of lysates from wild type (WT) and Edf1 knockout (KO) NIH/3T3 cells, using [KO Validated] Edf1 Rabbit pAb (STJ11106614) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/185810/407715/STJ11106614_1__42693.1744894238.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Edf1 knockout (KO) NIH/3T3 cells, using [KO Validated] Edf1 Rabbit pAb (STJ11106614) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.")
