Western blot analysis of lysates from mouse kidney, using EARS2 Rabbit polyclonal antibody (STJ117158) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-EARS2 antibody (42-220) (STJ117158)

SKU:
STJ117158
£110.50 - £505.50
Free Shipping
Processing The item has been added
Enquire For Bulk Order

Covered by St John's Laboratory Guarantee

Applications:WB/ELISA
Host:Rabbit
Reactivity:Mouse
Note:STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality :Polyclonal
Isotype:IgG
Conjugation:Unconjugated
Concentration:Lot specific
Purification:Affinity purification
Dilution Range:WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Formulation:PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Storage Instruction:Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol:EARS2
Gene ID:124454
Uniprot ID:SYEM_HUMAN
Immunogen Region:42-220
Immunogen Sequence:APSPTGFLHLGGLRTALYNY IFAKKYQGSFILRLEDTDQT RVVPGAAENIEDMLEWAGIP PDESPRRGGPAGPYQQSQRL ELYAQATEALLKTGAAYPCF CSPQRLELLKKEALRNHQTP RYDNRCRNMSQEQVAQKLAK DPKPAIRFRLEQVVPAFQDL VYGWNRHEVASVEGDPVIM
Specificity:Recombinant fusion protein containing a sequence corresponding to amino acids 42-220 of human EARS2 (NP_001295140.1).
Function Non-discriminating glutamyl-tRNA synthetase that catalyzes aminoacylation of both mitochondrial tRNA(Glu) and tRNA(Gln) and participates in RNA aminoacylation for mitochondrial protein translation. Attachs glutamate to tRNA(Glu) or tRNA(Gln) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) or tRNA(Gln). In vitro, cytoplasmic tRNA(Gln) is slightly glutamylated, but with low activity.
Protein Name Nondiscriminating Glutamyl-Trna Synthetase Ears2 - Mitochondrial
Glutamate--Trna(Gln Ligase Ears2 - Mitochondrial
Glutamyl-Trna Synthetase
Glurs
Mitochondrial Glutamyl-Trna Synthetase
Mtglurs
Database Links Reactome: R-HSA-379726
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Nondiscriminating Glutamyl-Trna Synthetase Ears2 - Mitochondrial antibody
Anti-Glutamate--Trna(Gln Ligase Ears2 - Mitochondrial antibody
Anti-Glutamyl-Trna Synthetase antibody
Anti-Glurs antibody
Anti-Mitochondrial Glutamyl-Trna Synthetase antibody
Anti-Mtglurs antibody
Anti-EARS2 antibody
Anti-KIAA1970 antibody

Information sourced from Uniprot.org

Related Products

Model Name Host Clonality Reactivity Application
STJA0008973 Anti-EARS2 antibody (STJA0008973) Rabbit Polyclonal Human/Mouse WB
STJ94197 Anti-MRGPRE antibody (171-220) (STJ94197) Rabbit Polyclonal Human/Mouse/Rat WB/ELISA/IHC
STJ502561 Anti-PMS2 antibody (220-270) (STJ502561) Rabbit Polyclonal Human/Mouse/Rat ELISA/IHC/IP/WB
STJ502960 Anti-SFN antibody (150-220) (STJ502960) Rabbit Polyclonal Human/Mouse/Rat ELISA/IP/WB
STJ500138 Anti-AQP6 antibody (220-270) (STJ500138) Rabbit Polyclonal Bovine/Human/Mouse/Rat/Sheep ELISA/IP/WB

Related Products

!