Western blot analysis of lysates from mouse kidney, using EARS2 Rabbit polyclonal antibody (STJ117158) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-EARS2 antibody (42-220) (STJ117158)

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STJ117158
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EARS2
Gene ID: 124454
Uniprot ID: SYEM_HUMAN
Immunogen Region: 42-220
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 42-220 of human EARS2 (NP_001295140.1).
Immunogen Sequence: APSPTGFLHLGGLRTALYNY IFAKKYQGSFILRLEDTDQT RVVPGAAENIEDMLEWAGIP PDESPRRGGPAGPYQQSQRL ELYAQATEALLKTGAAYPCF CSPQRLELLKKEALRNHQTP RYDNRCRNMSQEQVAQKLAK DPKPAIRFRLEQVVPAFQDL VYGWNRHEVASVEGDPVIM
Function Non-discriminating glutamyl-tRNA synthetase that catalyzes aminoacylation of both mitochondrial tRNA(Glu) and tRNA(Gln) and participates in RNA aminoacylation for mitochondrial protein translation. Attachs glutamate to tRNA(Glu) or tRNA(Gln) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) or tRNA(Gln). In vitro, cytoplasmic tRNA(Gln) is slightly glutamylated, but with low activity.
Protein Name Nondiscriminating Glutamyl-Trna Synthetase Ears2 - Mitochondrial
Glutamate--Trna(Gln Ligase Ears2 - Mitochondrial
Glutamyl-Trna Synthetase
Glurs
Mitochondrial Glutamyl-Trna Synthetase
Mtglurs
Database Links Reactome: R-HSA-379726
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Nondiscriminating Glutamyl-Trna Synthetase Ears2 - Mitochondrial antibody
Anti-Glutamate--Trna(Gln Ligase Ears2 - Mitochondrial antibody
Anti-Glutamyl-Trna Synthetase antibody
Anti-Glurs antibody
Anti-Mitochondrial Glutamyl-Trna Synthetase antibody
Anti-Mtglurs antibody
Anti-EARS2 antibody
Anti-KIAA1970 antibody

Information sourced from Uniprot.org

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