| Host: | Rabbit |
| Applications: | WB/IP/ELISA/ChIP |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:100-1:500IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.ChIP:5 Mu g antib |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | E2F6 |
| Gene ID: | 1876 |
| Uniprot ID: | E2F6_HUMAN |
| Immunogen Region: | 1-281 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-281 of human E2F6 (NP_937987.2). |
| Immunogen Sequence: | MSQQRPARKLPSLLLDPTEE TVRRRCRDPINVEGLLPSKI RINLEDNVQYVSMRKALKVK RPRFDVSLVYLTRKFMDLVR SAPGGILDLNKVATKLGVRK RRVYDITNVLDGIDLVEKKS KNHIRWIGSDLSNFGAVPQQ KKLQEELSDLSAMEDALDEL IKDCAQQLFELTDDKENERL AYVTYQDIHSIQAFHEQIVI AVKAPAETRLDVPAPREDSI TVHIRSTNGPIDVYLCEVE |
| Tissue Specificity | Expressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen. |
| Function | Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTCCGCGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. Represses expression of some meiosis-specific genes, including SLC25A31/ANT4. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase. |
| Protein Name | Transcription Factor E2f6E2f-6 |
| Database Links | Reactome: R-HSA-69205Reactome: R-HSA-8953750 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Transcription Factor E2f6 antibodyAnti-E2f-6 antibodyAnti-E2F6 antibody |
Information sourced from Uniprot.org