• Western blot analysis of extracts of various cell lines, using E2F3 antibody (STJ111428) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of various lysates using E2F3 Rabbit polyclonal antibody (STJ111428) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-E2F3 antibody (200-300) (STJ111428)

SKU:
STJ111428

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-E2F3 (200-300) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: E2F3
Gene ID: 1871
Uniprot ID: E2F3_HUMAN
Immunogen Region: 200-300
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human E2F3 (NP_001230005.1).
Immunogen Sequence: SIESLQIHLASTQGPIEVYL CPEETETHSPMKTNNQDHNG NIPKPASKDLASTNSGHSDC SVSMGNLSPLASPANLLQQT EDQIPSNLEGPFVNLLPPLL Q
Function Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTCCGCGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner. Inhibits adipogenesis, probably through the repression of CEBPA binding to its target gene promoters.
Protein Name Transcription Factor E2f3
E2f-3
Database Links Reactome: R-HSA-1912408
Reactome: R-HSA-2559580
Reactome: R-HSA-2559585
Reactome: R-HSA-68911
Reactome: R-HSA-69231
Reactome: R-HSA-9661069
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor E2f3 antibody
Anti-E2f-3 antibody
Anti-E2F3 antibody
Anti-KIAA0075 antibody

Information sourced from Uniprot.org

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