| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: | Monoclonal |
| Clone ID: | SRM |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:1000-1:4000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Gene Symbol: | CDH1 |
| Gene ID: | 999 |
| Uniprot ID: | CADH1_HUMAN |
| Immunogen Region: | 775-874 aa |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 775-874 of human E-Cadherin (NP_004351.1). |
| Immunogen Sequence: | GLDARPEVTRNDVAPTLMSV PRYLPRPANPDEIGNFIDEN LKAADTDPTAPPYDSLLVFD YEGSGSEAASLSSLNSSESD KDQDYDYLNEWGNRFKKLAD |
| Tissue Specificity | Expressed in granuloma macrophages (at protein level). Expressed in the skin (at protein level). Expressed in the liver. |
| Post Translational Modifications | During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions. During development of the cochlear organ of Corti, cleavage by ADAM10 at adherens junctions promotes pillar cell separation. N-glycosylation at Asn-637 is essential for expression, folding and trafficking. Addition of bisecting N-acetylglucosamine by MGAT3 modulates its cell membrane location. Ubiquitinated by a SCF complex containing SKP2, which requires prior phosphorylation by CK1/CSNK1A1. Ubiquitinated by CBLL1/HAKAI, requires prior phosphorylation at Tyr-754. O-glycosylated. O-manosylated by TMTC1, TMTC2, TMTC3 or TMTC4. Thr-285 and Thr-509 are O-mannosylated by TMTC2 or TMTC4 but not TMTC1 or TMTC3. (Microbial infection) Cleaved by S.pyogenes SpeB protease.leading to its degradation. Degradation by SpeB promotes bacterial translocation across the host epithelial barrier. |
| Function | Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells.cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production. (Microbial infection) Serves as a receptor for Listeria monocytogenes.internalin A (InlA) binds to this protein and promotes uptake of the bacteria. |
| Protein Name | Cadherin-1 Cam 120/80 Epithelial Cadherin E-Cadherin Uvomorulin Cd Antigen Cd324 Cleaved Into - E-Cad/Ctf1 - E-Cad/Ctf2 - E-Cad/Ctf3 |
| Database Links | Reactome: R-HSA-1474228 Reactome: R-HSA-198933 Reactome: R-HSA-216083 Reactome: R-HSA-351906 Reactome: R-HSA-418990 Reactome: R-HSA-5626467 Reactome: R-HSA-8876493 Reactome: R-HSA-9823730 Reactome: R-HSA-9856649 |
| Cellular Localisation | Cell Junction Adherens Junction Cell Membrane Single-Pass Type I Membrane Protein Endosome Golgi Apparatus Trans-Golgi Network Cytoplasm Desmosome Colocalizes With Dlgap5 At Sites Of Cell-Cell Contact In Intestinal Epithelial Cells Anchored To Actin Microfilaments Through Association With Alpha- Beta- And Gamma-Catenin Sequential Proteolysis Induced By Apoptosis Or Calcium Influx Results In Translocation From Sites Of Cell-Cell Contact To The Cytoplasm Colocalizes With Rab11a Endosomes During Its Transport From The Golgi Apparatus To The Plasma Membrane Recruited To Desmosomes At The Initial Assembly Phase And Also Accumulates Progressively At Mature Desmosome Cell-Cell Junctions Localizes To Cell-Cell Contacts As Keratinocyte Differentiation Progresses |
| Alternative Antibody Names | Anti-Cadherin-1 antibody Anti-Cam 120/80 antibody Anti-Epithelial Cadherin antibody Anti-E-Cadherin antibody Anti-Uvomorulin antibody Anti-Cd Antigen Cd324 Cleaved Into - E-Cad/Ctf1 - E-Cad/Ctf2 - E-Cad/Ctf3 antibody Anti-CDH1 antibody Anti-CDHE antibody Anti-UVO antibody |
Information sourced from Uniprot.org