| Host: |
Rabbit |
| Applications: |
WB/IHC-P/ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Monoclonal |
| Clone ID: |
S1MR |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DYSF |
| Gene ID: |
8291 |
| Uniprot ID: |
DYSF_HUMAN |
| Immunogen Region: |
50-150 |
| Specificity: |
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Dysferlin (Romeo) (O75923). |
| Immunogen Sequence: |
FEWDLKGIPLDQGSELHVVV KDHETMGRNRFLGEAKVPLR EVLATPSLSASFNAPLLDTK KQPTGASLVLQVSYTPLPGA VPLFPPPTPLEPSPTLPDLD V |
| Tissue Specificity | Expressed in skeletal muscle, myoblast, myotube and in the syncytiotrophoblast (STB) of the placenta (at protein level). Ubiquitous. Highly expressed in skeletal muscle. Also found in heart, brain, spleen, intestine, placenta and at lower levels in liver, lung, kidney and pancreas. |
| Function | Key calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress. |
| Protein Name | DysferlinDystrophy-Associated Fer-1-Like ProteinFer-1-Like Protein 1 |
| Database Links | Reactome: R-HSA-445355 |
| Cellular Localisation | Cell MembraneSarcolemmaSingle-Pass Type Ii Membrane ProteinCytoplasmic Vesicle MembraneColocalizesDuring Muscle DifferentiationWith Bin1 In The T-Tubule System Of Myotubules And At The Site Of Contact Between Two Myotubes Or A Myoblast And A MyotubeWounding Of Myotubes Led To Its Focal Enrichment To The Site Of Injury And To Its Relocalization In A Ca(2+)-Dependent Manner Toward The Plasma MembraneColocalizes With AhnakAhnak2 And Parvb At The Sarcolemma Of Skeletal MuscleDetected On The Apical Plasma Membrane Of The SyncytiotrophoblastReaches The Plasmma Membrane Through A Caveolin-Independent MechanismRetained By Caveolin At The Plasmma MembraneWith Cacna1s In The T-Tubule System Of MyotubulesAccumulates And Colocalizes With Fusion Vesicles At The Sarcolemma Disruption Sites |
| Alternative Antibody Names | Anti-Dysferlin antibodyAnti-Dystrophy-Associated Fer-1-Like Protein antibodyAnti-Fer-1-Like Protein 1 antibodyAnti-DYSF antibodyAnti-FER1L1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of various lysates using [KO Validated] IRF3 Rabbit monoclonal antibody (STJ11101781) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/90696/360781/STJ11101781_1__59011.1713125500.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] IRF3 Rabbit monoclonal antibody (STJ11101781) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
