• STJ70251 (1µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70251 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70251 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70251 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-Dynactin antibody (C-Term) (STJ70251)

SKU:
STJ70251-100

$409.73
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Host: Goat
Applications: Pep-ELISA/WB/IF/IHC/FC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Dynactin (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence, Immunohistochemistry and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-2-4µg/ml
IF-Strong expression of the protein seen in the cytoplasm of HeLa cells and additionally nucleus of U2OS cells. 10µg/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: DCTN1
Gene ID: 1639
Uniprot ID: DCTN1_HUMAN
Immunogen Region: C-Term
Accession Number: NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1; NP_001177765.1; NP_001177766.1
Specificity: This antibody is expected to recognise all reported isoforms.
Immunogen Sequence: QEQLHQLHSRLIS
Post Translational Modifications Ubiquitinated by a SCF complex containing FBXL5, leading to its degradation by the proteasome. Phosphorylation by SLK at Thr-145, Thr-146 and Thr-147 targets DCTN1 to the centrosome. It is uncertain if SLK phosphorylates all three threonines or one or two of them. PLK1-mediated phosphorylation at Ser-179 is essential for its localization in the nuclear envelope, promotes its dissociation from microtubules during early mitosis and positively regulates nuclear envelope breakdown during prophase.
Function Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules. Plays a key role in dynein-mediated retrograde transport of vesicles and organelles along microtubules by recruiting and tethering dynein to microtubules. Binds to both dynein and microtubules providing a link between specific cargos, microtubules and dynein. Essential for targeting dynein to microtubule plus ends, recruiting dynein to membranous cargos and enhancing dynein processivity (the ability to move along a microtubule for a long distance without falling off the track). Can also act as a brake to slow the dynein motor during motility along the microtubule. Can regulate microtubule stability by promoting microtubule formation, nucleation and polymerization and by inhibiting microtubule catastrophe in neurons. Inhibits microtubule catastrophe by binding both to microtubules and to tubulin, leading to enhanced microtubule stability along the axon. Plays a role in metaphase spindle orientation. Plays a role in centriole cohesion and subdistal appendage organization and function. Its recruitment to the centriole in a KIF3A-dependent manner is essential for the maintenance of centriole cohesion and the formation of subdistal appendage. Also required for microtubule anchoring at the mother centriole. Plays a role in primary cilia formation.
Protein Name Dynactin Subunit 1
150 Kda Dynein-Associated Polypeptide
Dap-150
Dp-150
P135
P150-Glued
Database Links Reactome: R-HSA-2132295
Reactome: R-HSA-2565942 Q14203-2
Reactome: R-HSA-3371497
Reactome: R-HSA-380259 Q14203-2
Reactome: R-HSA-380270 Q14203-2
Reactome: R-HSA-380284 Q14203-2
Reactome: R-HSA-380320 Q14203-2
Reactome: R-HSA-381038
Reactome: R-HSA-5620912 Q14203-2
Reactome: R-HSA-6807878
Reactome: R-HSA-6811436
Reactome: R-HSA-8854518 Q14203-2
Reactome: R-HSA-9725370
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Centriole
Spindle
Nucleus Envelope
Cell Cortex
Localizes To Microtubule Plus Ends
Localizes Preferentially To The Ends Of Tyrosinated Microtubules
Localization At Centrosome Is Regulated By Slk-Dependent Phosphorylation
Localizes To Centrosome In A Parkda-Dependent Manner
Localizes To The Subdistal Appendage Region Of The Centriole In A Kif3a-Dependent Manner
Plk1-Mediated Phosphorylation At Ser-179 Is Essential For Its Localization In The Nuclear Envelope
Alternative Antibody Names Anti-Dynactin Subunit 1 antibody
Anti-150 Kda Dynein-Associated Polypeptide antibody
Anti-Dap-150 antibody
Anti-Dp-150 antibody
Anti-P135 antibody
Anti-P150-Glued antibody
Anti-DCTN1 antibody

Information sourced from Uniprot.org

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