• Biotinylated STJ70779 (0. 1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-Duffy/FY/DARC, Biotinylated antibody (N-Term) {Biotin} (STJ73201)

SKU:
STJ73201-100

$409.73
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Host: Goat
Applications: Pep-ELISA/WB/IHC/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Duffy/FY/DARC, Biotinylated (N-Term) is suitable for use in ELISA, Western Blot, Immunohistochemistry and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Biotin
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.1-0.3µg/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: ACKR1
Gene ID: 2532
Uniprot ID: ACKR1_HUMAN
Immunogen Region: N-Term
Accession Number: NP_001116423.1; NP_002027.2
Specificity: This antibody is expected to recognize both reported isoforms (NP_001116423.1; NP_002027.2).
Immunogen Sequence: HRAELSPSTENSSQLDFEDC.
Function Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Has a promiscuous chemokine-binding profile, interacting with inflammatory chemokines of both the CXC and the CC subfamilies but not with homeostatic chemokines. Acts as a receptor for chemokines including CCL2, CCL5, CCL7, CCL11, CCL13, CCL14, CCL17, CXCL5, CXCL6, IL8/CXCL8, CXCL11, GRO, RANTES, MCP-1, TARC and also for the malaria parasites P.vivax and P.knowlesi. May regulate chemokine bioavailability and, consequently, leukocyte recruitment through two distinct mechanisms: when expressed in endothelial cells, it sustains the abluminal to luminal transcytosis of tissue-derived chemokines and their subsequent presentation to circulating leukocytes.when expressed in erythrocytes, serves as blood reservoir of cognate chemokines but also as a chemokine sink, buffering potential surges in plasma chemokine levels.
Protein Name Atypical Chemokine Receptor 1
Duffy Antigen/Chemokine Receptor
Fy Glycoprotein
Gpfy
Glycoprotein D
Plasmodium Vivax Receptor
Cd Antigen Cd234
Database Links Reactome: R-HSA-375276
Cellular Localisation Early Endosome
Recycling Endosome
Membrane
Multi-Pass Membrane Protein
Predominantly Localizes To Endocytic Vesicles
And Upon Stimulation By The Ligand Is Internalized Via Caveolae
Once Internalized
The Ligand Dissociates From The Receptor
And Is Targeted To Degradation While The Receptor Is Recycled Back To The Cell Membrane
Alternative Antibody Names Anti-Atypical Chemokine Receptor 1 antibody
Anti-Duffy Antigen/Chemokine Receptor antibody
Anti-Fy Glycoprotein antibody
Anti-Gpfy antibody
Anti-Glycoprotein D antibody
Anti-Plasmodium Vivax Receptor antibody
Anti-Cd Antigen Cd234 antibody
Anti-ACKR1 antibody
Anti-DARC antibody
Anti-FY antibody
Anti-GPD antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance