• Immunohistochemistry analysis of DTX2 in paraffin-embedded mouse liver using DTX2 Rabbit polyclonal antibody (STJ29535) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of DTX2 in paraffin-embedded human kidney using DTX2 Rabbit polyclonal antibody (STJ29535) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of DTX2 in paraffin-embedded rat spleen using DTX2 Rabbit polyclonal antibody (STJ29535) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of DTX2 in paraffin-embedded rat liver using DTX2 Rabbit polyclonal antibody (STJ29535) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from A-549 cells, using DTX2 Rabbit polyclonal antibody (STJ29535) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-DTX2 antibody (1-280) (STJ29535)

SKU:
STJ29535

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DTX2 (1-280) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DTX2
Gene ID: 113878
Uniprot ID: DTX2_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human DTX2 (NP_065943.2).
Immunogen Sequence: MAMAPSPSLVQVYTSPAAVA VWEWQDGLGTWHPYSATVCS FIEQQFVQQKGQRFGLGSLA HSIPLGQADPSLAPYIIDLP SWTQFRQDTGTMRAVRRHLF PQHSAPGRGVVWEWLSDDGS WTAYEASVCDYLEQQVARGN QLVDLAPLGYNYTVNYTTHT QTNKTSSFCRSVRRQAGPPY PVTTIIAPPGHTGVACSCHQ CLSGSRTGPVSGRYRHSMTN LPAYPVPQHPPHRTASVFG
Function Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Functions as a ubiquitin ligase protein in vitro, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity.
Protein Name Probable E3 Ubiquitin-Protein Ligase Dtx2
Protein Deltex-2
Deltex2
Hdtx2
Ring Finger Protein 58
Ring-Type E3 Ubiquitin Transferase Dtx2
Database Links Reactome: R-HSA-2122948
Cellular Localisation Cytoplasm
Nucleus
Predominantly Cytoplasmic
Partially Nuclear
Alternative Antibody Names Anti-Probable E3 Ubiquitin-Protein Ligase Dtx2 antibody
Anti-Protein Deltex-2 antibody
Anti-Deltex2 antibody
Anti-Hdtx2 antibody
Anti-Ring Finger Protein 58 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Dtx2 antibody
Anti-DTX2 antibody
Anti-KIAA1528 antibody
Anti-RNF58 antibody

Information sourced from Uniprot.org

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