• Immunofluorescence analysis of Dental pulp stem cell using DSPP Rabbit polyclonal antibody (STJ11105632) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from wild type (WT) and 293F cells transfected with DSPP using DSPP Rabbit polyclonal antibody (STJ11105632) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 40. 5  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.

Anti-DSPP antibody (16-230) (STJ11105632)

SKU:
STJ11105632

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Dentin Sialophosphoprotein Cleaved Into-Dentin Phosphoprotein (16-230) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DSPP
Gene ID: 1834
Uniprot ID: DSPP_HUMAN
Immunogen Region: 16-230
Immunogen: Recombinant Protein corresponding to a sequence within amino acids 16-230 of human DSPP (NP_055023.2).
Immunogen Sequence: IPVPQSKPLERHVEKSMNLH LLARSNVSVQDELNASGTIK ESGVLVHEGDRGRQENTQDG HKGEGNGSKWAEVGGKSFST YSTLANEEGNIEGWNGDTGK AETYGHDGIHGKEENITANG IQGQVSIIDNAGATNRSNTN GNTDKNTQNGDVGDAGHNED VAVVQEDGPQVAGSNNSTDN EDEIIENSCRNEGNTSEITP QINSKRNGTKEAEVT
Tissue Specificity Expressed in teeth. DPP is synthesized by odontoblast and transiently expressed by pre-ameloblasts.
Post Translational Modifications DSP is glycosylated.
Function DSP may be an important factor in dentinogenesis. DPP may bind high amount of calcium and facilitate initial mineralization of dentin matrix collagen as well as regulate the size and shape of the crystals.
Protein Name Dentin Sialophosphoprotein Cleaved Into - Dentin Phosphoprotein
Dentin Phosphophoryn
Dpp - Dentin Sialoprotein
Dsp
Database Links Reactome: R-HSA-3000178
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Dentin Sialophosphoprotein Cleaved Into - Dentin Phosphoprotein antibody
Anti-Dentin Phosphophoryn antibody
Anti-Dpp - Dentin Sialoprotein antibody
Anti-Dsp antibody
Anti-DSPP antibody

Information sourced from Uniprot.org

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