Anti-DSP antibody (1-20) (STJ29950)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Desmoplakin (1-20) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DSP |
| Gene ID | 1832 |
| Uniprot ID | DESP_HUMAN |
| Immunogen Region | 1-20 |
| Immunogen Sequence | MSCNGGSHPRINTLGRMIRA ESGPDLRYEVTSGGGGTSRM YYSRRGVITDQNSDGYCQTG TMSRHQNQNTIQELLQNCSD CLMRAELIVQPELKYGDGIQ |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Desmoplakin (NP_004406.2). |
Additional Info
| Tissue Specificity | Expressed in oral mucosa (at protein level). Expressed in arrector pili muscle (at protein level). Expressed in the heart in the heart (at protein level). Isoform DPI: Apparently an obligate constituent of all desmosomes. Isoform DPII: Resides predominantly in tissues and cells of stratified origin. |
| Post Translational Modifications | Phosphorylation at Ser-2849 increases association with intermediate filament cytokeratin, potentially facilitating interaction between desmosome junctions and intermediate filament architecture. |
| Function | Major high molecular weight protein of desmosomes. Regulates profibrotic gene expression in cardiomyocytes via activation of the MAPK14/p38 MAPK signaling cascade and increase in TGFB1 protein abundance. |
| Protein Name | DesmoplakinDp250/210 Kda Paraneoplastic Pemphigus Antigen |
| Database Links | Reactome: R-HSA-351906Reactome: R-HSA-6798695Reactome: R-HSA-6805567Reactome: R-HSA-6809371Reactome: R-HSA-9696264Reactome: R-HSA-9696273 |
| Cellular Localisation | Cell JunctionDesmosomeCell MembraneCytoplasmLocalizes To Desmosome Precursor Particles In The CytoplasmLocalizes To The Cytoplasm In Undifferentiated Keratinocytes However Becomes Localizes To Both Lateral And Tricellular Cell-Cell Contacts As Differentiation Progresses And As Epithelial Sheet Formation Completes |
| Alternative Antibody Names | Anti-Desmoplakin antibodyAnti-Dp antibodyAnti-250/210 Kda Paraneoplastic Pemphigus Antigen antibodyAnti-DSP antibody |
Information sourced from Uniprot.org