• Western blot analysis of extracts of various cell lines, using Dot1L/KMT4 antibody (STJ113756) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-DOT1L antibody (250-500) (STJ113756)

SKU:
STJ113756

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Dot1L/KMT4 (250-500) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DOT1L
Gene ID: 84444
Uniprot ID: DOT1L_HUMAN
Immunogen Region: 250-500
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 250-500 of human Dot1L/KMT4 (NP_115871.1).
Immunogen Sequence: DHQLKERFANMKEGGRIVSS KPFAPLNFRINSRNLSDIGT IMRVVELSPLKGSVSWTGKP VSYYLHTIDRTILENYFSSL KNPKLREEQEAARRRQQRES KSNAATPTKGPEGKVAGPAD APMDSGAEEEKAGAATVKKP SPSKARKKKLNKKGRKMAGR KRGRPKKMNTANPERKPKKN QTALDALHAQTVSQTAASSP QDAYRSPHSPFYQLPPSVQR HSPNPLLVAPTPPALQKLL
Function Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.
Protein Name Histone-Lysine N-Methyltransferase - H3 Lysine-79 Specific
Dot1-Like Protein
Histone H3-K79 Methyltransferase
H3-K79-Hmtase
Lysine N-Methyltransferase 4
Database Links Reactome: R-HSA-3214841
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Histone-Lysine N-Methyltransferase - H3 Lysine-79 Specific antibody
Anti-Dot1-Like Protein antibody
Anti-Histone H3-K79 Methyltransferase antibody
Anti-H3-K79-Hmtase antibody
Anti-Lysine N-Methyltransferase 4 antibody
Anti-DOT1L antibody
Anti-KIAA1814 antibody
Anti-KMT4 antibody

Information sourced from Uniprot.org

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