Anti-DOK3 antibody (101-150 aa) (STJ92762)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human DOK3 at the amino acid range 101-150
General Information
| Short Description | Rabbit polyclonal anti-Docking protein 3 (101-150 aa) for use in WB, IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DOK3 |
| Gene ID | 79930 |
| Uniprot ID | DOK3_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human DOK3 at the amino acid range 101-150 |
| Immunogen Region | 101-150 aa |
| Specificity | Dok-3 Polyclonal Antibody detects endogenous levels of Dok-3 protein. |
Additional Info
| Post Translational Modifications | Constitutively tyrosine-phosphorylated. On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL1 kinase. |
| Function | DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate ABL1 function. |
| Protein Name | Docking Protein 3Downstream Of Tyrosine Kinase 3 |
| Database Links | Reactome: R-HSA-6798695 |
| Cellular Localisation | CytoplasmCell MembranePeripheral Membrane ProteinCytoplasmic Side |
| Alternative Antibody Names | Anti-Docking Protein 3 antibodyAnti-Downstream Of Tyrosine Kinase 3 antibodyAnti-DOK3 antibody |
Information sourced from Uniprot.org