Anti-DOCK2 antibody (1551-1830) (STJ111190)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal DOCK2 (1551-1830) antibody for use in WB, IF, ICC, IP and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DOCK2 |
| Gene ID | 1794 |
| Uniprot ID | DOCK2_HUMAN |
| Immunogen Region | 1551-1830 |
| Immunogen Sequence | FTEEYVRDHPEDQDKLTHLK DLIAWQIPFLGAGIKIHEKR VSDNLRPFHDRMEECFKNLK MKVEKEYGVREMPDFDDRRV GRPRSMLRSYRQMSIISLAS MNSDCSTPSKPTSESFDLEL ASPKTPRVEQEEPISPGSTL PEVKLRRSKKRTKRSSVVFA DEKAAAESDLKRLSRKHEFM SDTNLSEHAAIPLKASVLSQ MSFASQSMPTIPALALSVAG IPGLDEANTSPRLSQTFLQ |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1551-1830 of human DOCK2 (NP_004937.1). |
Additional Info
| Tissue Specificity | Specifically expressed in hematopoietic cells. Highly expressed in peripheral blood leukocytes, and expressed at intermediate level in thymus and spleen. Expressed at very low level in the small intestine and colon. |
| Function | Involved in cytoskeletal rearrangements required for lymphocyte migration in response of chemokines. Activates RAC1 and RAC2, but not CDC42, by functioning as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. May also participate in IL2 transcriptional activation via the activation of RAC2. |
| Protein Name | Dedicator Of Cytokinesis Protein 2 |
| Database Links | Reactome: R-HSA-164944Reactome: R-HSA-6798695Reactome: R-HSA-8980692Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013408Reactome: R-HSA-983231 |
| Cellular Localisation | Endomembrane SystemPeripheral Membrane ProteinCytoplasmCytoskeletonColocalizes With F-Actin |
| Alternative Antibody Names | Anti-Dedicator Of Cytokinesis Protein 2 antibodyAnti-DOCK2 antibodyAnti-KIAA0209 antibody |
Information sourced from Uniprot.org