• Immunoprecipitation analysis of 300 Mu g extracts of Jurkat cells using 3 Mu g DOCK2 antibody (STJ111190). Western blot was performed from the immunoprecipitate using DOCK2 antibody (STJ111190) at a dilution of 1:500.
  • Immunofluorescence analysis of L929 cells using DOCK2 Rabbit polyclonal antibody (STJ111190) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Jurkat cells, using DOCK2 Rabbit polyclonal antibody (STJ111190). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-DOCK2 antibody (1551-1830) (STJ111190)

SKU:
STJ111190

£112.50 - £522.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DOCK2 (1551-1830) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
IP 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DOCK2
Gene ID: 1794
Uniprot ID: DOCK2_HUMAN
Immunogen Region: 1551-1830
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1551-1830 of human DOCK2 (NP_004937.1).
Immunogen Sequence: FTEEYVRDHPEDQDKLTHLK DLIAWQIPFLGAGIKIHEKR VSDNLRPFHDRMEECFKNLK MKVEKEYGVREMPDFDDRRV GRPRSMLRSYRQMSIISLAS MNSDCSTPSKPTSESFDLEL ASPKTPRVEQEEPISPGSTL PEVKLRRSKKRTKRSSVVFA DEKAAAESDLKRLSRKHEFM SDTNLSEHAAIPLKASVLSQ MSFASQSMPTIPALALSVAG IPGLDEANTSPRLSQTFLQ
Tissue Specificity Specifically expressed in hematopoietic cells. Highly expressed in peripheral blood leukocytes, and expressed at intermediate level in thymus and spleen. Expressed at very low level in the small intestine and colon.
Function Involved in cytoskeletal rearrangements required for lymphocyte migration in response of chemokines. Activates RAC1 and RAC2, but not CDC42, by functioning as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. May also participate in IL2 transcriptional activation via the activation of RAC2.
Protein Name Dedicator Of Cytokinesis Protein 2
Database Links Reactome: R-HSA-164944
Reactome: R-HSA-6798695
Reactome: R-HSA-8980692
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013408
Reactome: R-HSA-983231
Cellular Localisation Endomembrane System
Peripheral Membrane Protein
Cytoplasm
Cytoskeleton
Colocalizes With F-Actin
Alternative Antibody Names Anti-Dedicator Of Cytokinesis Protein 2 antibody
Anti-DOCK2 antibody
Anti-KIAA0209 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance