| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-DNASE1 (22-282) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DNASE1 |
| Gene ID: |
1773 |
| Uniprot ID: |
DNAS1_HUMAN |
| Immunogen Region: |
22-282 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 22-282 of human DNASE1 (NP_005214.2). |
| Immunogen Sequence: |
SLKIAAFNIQTFGETKMSNA TLVSYIVQILSRYDIALVQE VRDSHLTAVGKLLDNLNQDA PDTYHYVVSEPLGRNSYKER YLFVYRPDQVSAVDSYYYDD GCEPCGNDTFNREPAIVRFF SRFTEVREFAIVPLHAAPGD AVAEIDALYDVYLDVQEKWG LEDVMLMGDFNAGCSYVRPS QWSSIRLWTSPTFQWLIPDS ADTTATPTHCAYDRIVVAGM LLRGAVVPDSALPFNFQAA |
| Tissue Specificity | Principally in tissues of the digestive system. Highest levels found in urine, but also relatively abundant in semen and saliva. |
| Function | Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs. Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA. Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation. Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation. |
| Protein Name | Deoxyribonuclease-1Deoxyribonuclease IDnase IDornase Alfa |
| Cellular Localisation | SecretedZymogen GranuleNucleus EnvelopeSecretory ProteinStored In Zymogen Granules And Found In The Nuclear Envelope |
| Alternative Antibody Names | Anti-Deoxyribonuclease-1 antibodyAnti-Deoxyribonuclease I antibodyAnti-Dnase I antibodyAnti-Dornase Alfa antibodyAnti-DNASE1 antibodyAnti-DNL1 antibodyAnti-DRNI antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and ESD knockout (KO) 293T cells, using [KO Validated] ESD Rabbit polyclonal antibody (STJ11100739) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89677/358170/STJ11100739_1__87167.1713122476.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ESD knockout (KO) 293T cells, using [KO Validated] ESD Rabbit polyclonal antibody (STJ11100739) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")