Anti-DNAJB6 antibody (177-326) (STJ29132)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal DNAJB6 (177-326) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DNAJB6 |
| Gene ID | 10049 |
| Uniprot ID | DNJB6_HUMAN |
| Immunogen Region | 177-326 |
| Immunogen Sequence | STSFGGSGMGNFKSISTSTK MVNGRKITTKRIVENGQERV EVEEDGQLKSLTINGVADDD ALAEERMRRGQNALPAQPAG LRPPKPPRPASLLRHAPHCL SEEEGEQDRPRAPGPWDPLA SAAGLKEGGKRKKQKQREES KKKKSTKGNH |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 177-326 of human DNAJB6 (NP_490647.1). |
Additional Info
| Tissue Specificity | Widely expressed. Highest levels in testis and brain, and lower levels in heart, spleen, intestine, ovary, placenta, lung, kidney, pancreas, thymus, prostate, skeletal muscle, liver and leukocytes. In testis, expressed in germ cells in the earlier stages of differentiation pathway as well as in spermatids. In brain, expressed at a higher level in hippocampus and thalamus and a lower level in amygdala, substantia nigra, corpus callosum and caudate nucleus. |
| Function | Has a stimulatory effect on the ATPase activity of HSP70 in a dose-dependent and time-dependent manner and hence acts as a co-chaperone of HSP70. Plays an indispensable role in the organization of KRT8/KRT18 filaments. Acts as an endogenous molecular chaperone for neuronal proteins including huntingtin. Suppresses aggregation and toxicity of polyglutamine-containing, aggregation-prone proteins. Also reduces cellular toxicity and caspase-3 activity. Isoform B: Isoform B but not isoform A inhibits huntingtin aggregation. |
| Protein Name | Dnaj Homolog Subfamily B Member 6Hhdj1Heat Shock Protein J2Hsj-2MrjMsj-1 |
| Database Links | Reactome: R-HSA-3371453 |
| Cellular Localisation | CytoplasmPerinuclear RegionNucleusMyofibrilSarcomereZ Line |
| Alternative Antibody Names | Anti-Dnaj Homolog Subfamily B Member 6 antibodyAnti-Hhdj1 antibodyAnti-Heat Shock Protein J2 antibodyAnti-Hsj-2 antibodyAnti-Mrj antibodyAnti-Msj-1 antibodyAnti-DNAJB6 antibodyAnti-HSJ2 antibodyAnti-MRJ antibodyAnti-MSJ1 antibody |
Information sourced from Uniprot.org