| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-DLD (36-300) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:100-1:500IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DLD |
| Gene ID: |
1738 |
| Uniprot ID: |
DLDH_HUMAN |
| Immunogen Region: |
36-300 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 36-300 of human DLDH/DLDH/DLDH/DLD (NP_000099.2). |
| Immunogen Sequence: |
ADQPIDADVTVIGSGPGGYV AAIKAAQLGFKTVCIEKNET LGGTCLNVGCIPSKALLNNS HYYHMAHGKDFASRGIEMSE VRLNLDKMMEQKSTAVKALT GGIAHLFKQNKVVHVNGYGK ITGKNQVTATKADGGTQVID TKNILIATGSEVTPFPGITI DEDTIVSSTGALSLKKVPEK MVVIGAGVIGVELGSVWQRL GADVTAVEFLGHVGGVGIDM EISKNFQRILQKQGFKFKL |
| Post Translational Modifications | Tyrosine phosphorylated. |
| Function | Lipoamide dehydrogenase is a component of the glycine cleavage system as well as an E3 component of three alpha-ketoacid dehydrogenase complexes (pyruvate-, alpha-ketoglutarate-, and branched-chain amino acid-dehydrogenase complex). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A. In monomeric form may have additional moonlighting function as serine protease. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction. |
| Protein Name | Dihydrolipoyl Dehydrogenase - MitochondrialDihydrolipoamide DehydrogenaseGlycine Cleavage System L Protein |
| Database Links | Reactome: R-HSA-204174Reactome: R-HSA-389661Reactome: R-HSA-5362517Reactome: R-HSA-6783984Reactome: R-HSA-70268Reactome: R-HSA-70895Reactome: R-HSA-71064Reactome: R-HSA-71403 |
| Cellular Localisation | Mitochondrion MatrixNucleusCell ProjectionCiliumFlagellumCytoplasmic VesicleSecretory VesicleAcrosomeMainly Localizes In The MitochondrionA Small Fraction Localizes To The NucleusWhere The 2-Oxoglutarate Dehydrogenase Complex Is Required For Histone Succinylation |
| Alternative Antibody Names | Anti-Dihydrolipoyl Dehydrogenase - Mitochondrial antibodyAnti-Dihydrolipoamide Dehydrogenase antibodyAnti-Glycine Cleavage System L Protein antibodyAnti-DLD antibodyAnti-GCSL antibodyAnti-LAD antibodyAnti-PHE3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
