• Immunofluorescence analysis of HeLa cells using DLDH/DLDH/DLDH/DLD antibody (STJ115260). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human placenta using DLDH/DLDH/DLDH/DLD Rabbit polyclonal antibody (STJ115260) at dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using DLDH/DLDH/DLDH/DLD Rabbit polyclonal antibody (STJ115260) at dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using DLDH/DLDH/DLDH/DLD antibody (STJ115260) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-DLD antibody (36-300) (STJ115260)

SKU:
STJ115260

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DLD (36-300) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DLD
Gene ID: 1738
Uniprot ID: DLDH_HUMAN
Immunogen Region: 36-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 36-300 of human DLDH/DLDH/DLDH/DLD (NP_000099.2).
Immunogen Sequence: ADQPIDADVTVIGSGPGGYV AAIKAAQLGFKTVCIEKNET LGGTCLNVGCIPSKALLNNS HYYHMAHGKDFASRGIEMSE VRLNLDKMMEQKSTAVKALT GGIAHLFKQNKVVHVNGYGK ITGKNQVTATKADGGTQVID TKNILIATGSEVTPFPGITI DEDTIVSSTGALSLKKVPEK MVVIGAGVIGVELGSVWQRL GADVTAVEFLGHVGGVGIDM EISKNFQRILQKQGFKFKL
Post Translational Modifications Tyrosine phosphorylated.
Function Lipoamide dehydrogenase is a component of the glycine cleavage system as well as an E3 component of three alpha-ketoacid dehydrogenase complexes (pyruvate-, alpha-ketoglutarate-, and branched-chain amino acid-dehydrogenase complex). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A. In monomeric form may have additional moonlighting function as serine protease. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.
Protein Name Dihydrolipoyl Dehydrogenase - Mitochondrial
Dihydrolipoamide Dehydrogenase
Glycine Cleavage System L Protein
Database Links Reactome: R-HSA-204174
Reactome: R-HSA-389661
Reactome: R-HSA-5362517
Reactome: R-HSA-6783984
Reactome: R-HSA-70268
Reactome: R-HSA-70895
Reactome: R-HSA-71064
Reactome: R-HSA-71403
Cellular Localisation Mitochondrion Matrix
Nucleus
Cell Projection
Cilium
Flagellum
Cytoplasmic Vesicle
Secretory Vesicle
Acrosome
Mainly Localizes In The Mitochondrion
A Small Fraction Localizes To The Nucleus
Where The 2-Oxoglutarate Dehydrogenase Complex Is Required For Histone Succinylation
Alternative Antibody Names Anti-Dihydrolipoyl Dehydrogenase - Mitochondrial antibody
Anti-Dihydrolipoamide Dehydrogenase antibody
Anti-Glycine Cleavage System L Protein antibody
Anti-DLD antibody
Anti-GCSL antibody
Anti-LAD antibody
Anti-PHE3 antibody

Information sourced from Uniprot.org

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