• Immunofluorescence analysis of U-2 OS cells using DKC1 Rabbit polyclonal antibody (STJ114780) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using DKC1 Rabbit polyclonal antibody (STJ114780) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of DKC1 in paraffin-embedded human lung cancer using DKC1 Rabbit polyclonal antibody (STJ114780) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using DKC1 Rabbit polyclonal antibody (STJ114780) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-DKC1 antibody (1-180) (STJ114780)

SKU:
STJ114780

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:100
IF/ICC:1:50-1:100
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DKC1
Gene ID: 1736
Uniprot ID: DKC1_HUMAN
Immunogen Region: 1-180
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human DKC1 (NP_001354.1).
Immunogen Sequence: MADAEVIILPKKHKKKKERK SLPEEDVAEIQHAEEFLIKP ESKVAKLDTSQWPLLLKNFD KLNVRTTHYTPLACGSNPLK REIGDYIRTGFINLDKPSNP SSHEVVAWIRRILRVEKTGH SGTLDPKVTGCLIVCIERAT RLVKSQQSAGKEYVGIVRLH NAIEGGTQLSRALETLTGAL
Tissue Specificity Ubiquitously expressed.
Function Isoform 1: Catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. Required for ribosome biogenesis and telomere maintenance. Also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme. Isoform 3: Promotes cell to cell and cell to substratum adhesion, increases the cell proliferation rate and leads to cytokeratin hyper-expression.
Protein Name H/Aca Ribonucleoprotein Complex Subunit Dkc1
Cbf5 Homolog
Dyskerin
Nopp140-Associated Protein Of 57 Kda
Nucleolar Protein Nap57
Nucleolar Protein Family A Member 4
Snornp Protein Dkc1
Database Links Reactome: R-HSA-171319
Reactome: R-HSA-6790901
Cellular Localisation Isoform 1: Nucleus
Nucleolus
Nucleus
Cajal Body
Isoform 3: Cytoplasm
Alternative Antibody Names Anti-H/Aca Ribonucleoprotein Complex Subunit Dkc1 antibody
Anti-Cbf5 Homolog antibody
Anti-Dyskerin antibody
Anti-Nopp140-Associated Protein Of 57 Kda antibody
Anti-Nucleolar Protein Nap57 antibody
Anti-Nucleolar Protein Family A Member 4 antibody
Anti-Snornp Protein Dkc1 antibody
Anti-DKC1 antibody
Anti-NOLA4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance