Anti-DIXDC1 antibody (Internal) (STJ72102)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-DIXDC1 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 60kDa band observed in Human Heart and Skeletal Muscle lysates (calculated MW of 53.9kDa according to NP_219493.1). Recommended concentration: 0.5-2ยตg/ml. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20ยฐC on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | DIXDC1 |
| Gene ID | 85458 |
| Uniprot ID | DIXC1_HUMAN |
| Accession Number | NP_001033043.1; NP_219493.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | DVSYHQVDLERE |
| Specificity | This antibody is expected to recognize both isoforms (NP_001033043.1; NP_219493.1). |
Additional Info
| Post Translational Modifications | Phosphorylated on tyrosine and serine residues. Polyubiquitinated, leading to its proteasomal degradation. WNT3A signaling increases DIXDC1 protein levels by inhibiting its ubiquitination and subsequent degradation. |
| Function | Positive effector of the Wnt signaling pathway.activates WNT3A signaling via DVL2. Regulates JNK activation by AXIN1 and DVL2. |
| Protein Name | DixinCoiled-Coil Protein Dix1Coiled-Coil-Dix1Dix Domain-Containing Protein 1 |
| Cellular Localisation | Cell JunctionFocal AdhesionCytoplasmCytoskeletonStress FiberColocalizes With Gamma-Tubulin At The CentrosomeBoth During Interphase And MitosisAssociated With Actin Stress Fiber At The Filament EndsIsoform 2: CytoplasmNot Associated With Stress Fibers |
| Alternative Antibody Names | Anti-Dixin antibodyAnti-Coiled-Coil Protein Dix1 antibodyAnti-Coiled-Coil-Dix1 antibodyAnti-Dix Domain-Containing Protein 1 antibodyAnti-DIXDC1 antibodyAnti-CCD1 antibodyAnti-KIAA1735 antibody |
Information sourced from Uniprot.org