Western blot analysis of extracts of various cell lines, using DIO3 antibody (STJ28980) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-DIO3 antibody (70-170) (STJ28980)

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STJ28980
£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DIO3
Gene ID: 1735
Uniprot ID: IOD3_HUMAN
Immunogen Region: 70-170
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 70-170 of human DIO3 (NP_001353.4).
Immunogen Sequence: HFLGRRRRGQPEPEVELNSE GEEVPPDDPPICVSDDNRLC TLASLKAVWHGQKLDFFKQA HEGGPAPNSEVVLPDGFQSQ HILDYAQGNRPLVLNFGSCT U
Tissue Specificity Expressed in placenta and several fetal tissues.
Function Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development.
Protein Name Thyroxine 5-Deiodinase
5diii
Dioiii
Type 3 Di
Type Iii Iodothyronine Deiodinase
Database Links Reactome: R-HSA-350864
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Endosome Membrane
Alternative Antibody Names Anti-Thyroxine 5-Deiodinase antibody
Anti-5diii antibody
Anti-Dioiii antibody
Anti-Type 3 Di antibody
Anti-Type Iii Iodothyronine Deiodinase antibody
Anti-DIO3 antibody
Anti-ITDI3 antibody
Anti-TXDI3 antibody

Information sourced from Uniprot.org

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