• STJ71102 Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
  • STJ71102 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71102 (2µg/ml) staining of PFA-fixed cryo-sectioned Mouse Hippocampus. Microwaved antigen retrieval with citrate buffer pH 4. 5, HRP-staining.

Anti-DIO2 antibody (Internal) (STJ71102)

SKU:
STJ71102-100

£303.50
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Host: Goat
Applications: Pep-ELISA/FC/IFIHC
Reactivity: Human/Mouse/Rat/Pig
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-DIO2 (Internal) is suitable for use in ELISA, Flow Cytometry and ImmunofluorescenceImmunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-2-3µg/ml
IF-Strong expression of the protein seen in the cytoplasm and nuclei of HeLa cells. 10µg/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: DIO2
Gene ID: 1734
Uniprot ID: IOD2_HUMAN
Immunogen Region: Internal
Accession Number: NP_000784.2; NP_054644.1; NP_001007024.1
Specificity: This antibody is expected to recognise both reported isoform a (NP_000784.2 and NP_054644.1) and isoform b (NP_001007024.1).
Immunogen Sequence: EVKKHQNQEDRC
Post Translational Modifications Ubiquitinated by MARCHF6, leading to its degradation by the proteasome. Deubiquitinated by USP20 and USP33.
Function Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development.
Protein Name Type Ii Iodothyronine Deiodinase
5dii
Dioii
Type 2 Di
Type-Ii 5'-Deiodinase
Database Links Reactome: R-HSA-350864
Cellular Localisation Membrane
Single-Pass Membrane Protein
Alternative Antibody Names Anti-Type Ii Iodothyronine Deiodinase antibody
Anti-5dii antibody
Anti-Dioii antibody
Anti-Type 2 Di antibody
Anti-Type-Ii 5'-Deiodinase antibody
Anti-DIO2 antibody
Anti-ITDI2 antibody
Anti-TXDI2 antibody

Information sourced from Uniprot.org

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