| Host: | Goat |
| Applications: | Pep-ELISA/FC/IF/IHC |
| Reactivity: | Human/Mouse/Rat/Pig |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-DIO2 (Internal) is suitable for use in ELISA, Flow Cytometry, Immunofluorescence and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000.IHC: Cryo-sectioned Mouse Brain (Hippocampus) shows staining in the granular layer of the dentate gyrus. Recommended concentration: 2-3µg/ml. Data obtained from customer.IF: Strong e |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | DIO2 |
| Gene ID: | 1734 |
| Uniprot ID: | IOD2_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_000784.2; NP_054644.1; NP_001007024.1 |
| Specificity: | This antibody is expected to recognise both reported isoform a (NP_000784.2 and NP_054644.1) and isoform b (NP_001007024.1). |
| Immunogen Sequence: | EVKKHQNQEDRC |
| Post Translational Modifications | Ubiquitinated by MARCHF6, leading to its degradation by the proteasome. Deubiquitinated by USP20 and USP33. |
| Function | Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development. |
| Protein Name | Type Ii Iodothyronine Deiodinase5diiDioiiType 2 DiType-Ii 5'-Deiodinase |
| Database Links | Reactome: R-HSA-350864Reactome: R-HSA-9844594 |
| Cellular Localisation | MembraneSingle-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Type Ii Iodothyronine Deiodinase antibodyAnti-5dii antibodyAnti-Dioii antibodyAnti-Type 2 Di antibodyAnti-Type-Ii 5'-Deiodinase antibodyAnti-DIO2 antibodyAnti-ITDI2 antibodyAnti-TXDI2 antibody |
Information sourced from Uniprot.org