| Host: |
Rabbit |
| Applications: |
WB/IHC/IP |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-DIABLO (56-239) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200IP 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DIABLO |
| Gene ID: |
56616 |
| Uniprot ID: |
DBLOH_HUMAN |
| Immunogen Region: |
56-239 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 56-239 of human Smac/Diablo (NP_063940.1). |
| Immunogen Sequence: |
AVPIAQKSEPHSLSSEALMR RAVSLVTDSTSTFLSQTTYA LIEAITEYTKAVYTLTSLYR QYTSLLGKMNSEEEDEVWQV IIGARAEMTSKHQEYLKLET TWMTAVGLSEMAAEAAYQTG ADQASITARNHIQLVKLQVE EVHQLSRKAETKLAEAQIEE LRQKTQEEGEERAESEQEAY LRED |
| Tissue Specificity | Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed. |
| Post Translational Modifications | Ubiquitinated by BIRC7/livin. The precursor form is proteolytically cleaved by mitochondrial processing peptidase MPP to remove the transit peptide and produce an intermediate form. This is then processed by PARL to produce the mature cleaved form which is released from mitochondria into the cytosol in apoptotic cells. |
| Function | Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3: Attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1: Defective in the capacity to down-regulate the XIAP/BIRC4 abundance. |
| Protein Name | Diablo Iap-Binding Mitochondrial ProteinDiablo Homolog - MitochondrialDirect Iap-Binding Protein With Low PiSecond Mitochondria-Derived Activator Of CaspaseSmac Cleaved Into - Diablo Iap-Binding Mitochondrial Protein - Cleaved Form |
| Database Links | Reactome: R-HSA-111457Reactome: R-HSA-111463Reactome: R-HSA-111464Reactome: R-HSA-111469Reactome: R-HSA-9627069 |
| Cellular Localisation | MitochondrionCytoplasmCytosolReleased Into The Cytosol In A Parl-Dependent Manner When Cells Undergo Apoptosis |
| Alternative Antibody Names | Anti-Diablo Iap-Binding Mitochondrial Protein antibodyAnti-Diablo Homolog - Mitochondrial antibodyAnti-Direct Iap-Binding Protein With Low Pi antibodyAnti-Second Mitochondria-Derived Activator Of Caspase antibodyAnti-Smac Cleaved Into - Diablo Iap-Binding Mitochondrial Protein - Cleaved Form antibodyAnti-DIABLO antibodyAnti-SMAC antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
