Home
Primary Antibodies
Polyclonal Antibodies
Anti-Di-Methyl-Histone H4-R3 antibody (2-19) (STJ23979)

Immunofluorescence analysis of 293T cells using Symmetric DiMethyl-Histone H4-R3 Rabbit polyclonal antibody (STJ23979). Blue: DAPI for nuclear staining.
Immunohistochemistry analysis of Symmetric DiMethyl-Histone H4-R3 in paraffin-embedded human colon carcinoma tissue using Symmetric DiMethyl-Histone H4-R3 Rabbit polyclonal antibody (STJ23979) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
Immunohistochemistry analysis of Symmetric DiMethyl-Histone H4-R3 in paraffin-embedded human colon tissue using Symmetric DiMethyl-Histone H4-R3 Rabbit polyclonal antibody (STJ23979) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
Immunohistochemistry analysis of Symmetric DiMethyl-Histone H4-R3 in paraffin-embedded rat liver tissue using Symmetric DiMethyl-Histone H4-R3 Rabbit polyclonal antibody (STJ23979) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
Immunohistochemistry analysis of Symmetric DiMethyl-Histone H4-R3 in paraffin-embedded rat brain tissue using Symmetric DiMethyl-Histone H4-R3 Rabbit polyclonal antibody (STJ23979) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
Immunohistochemistry analysis of Symmetric DiMethyl-Histone H4-R3 in paraffin-embedded human spleen tissue using Symmetric DiMethyl-Histone H4-R3 Rabbit polyclonal antibody (STJ23979) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
Immunohistochemistry analysis of Symmetric DiMethyl-Histone H4-R3 in paraffin-embedded mouse brain tissue using Symmetric DiMethyl-Histone H4-R3 Rabbit polyclonal antibody (STJ23979) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
Immunohistochemistry analysis of Symmetric DiMethyl-Histone H4-R3 in paraffin-embedded human cervix cancer tissue using Symmetric DiMethyl-Histone H4-R3 Rabbit polyclonal antibody (STJ23979) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
Immunohistochemistry analysis of Symmetric DiMethyl-Histone H4-R3 in paraffin-embedded human liver tissue using Symmetric DiMethyl-Histone H4-R3 Rabbit polyclonal antibody (STJ23979) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
Immunohistochemistry analysis of Symmetric DiMethyl-Histone H4-R3 in paraffin-embedded mouse spleen tissue using Symmetric DiMethyl-Histone H4-R3 Rabbit polyclonal antibody (STJ23979) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
Immunohistochemistry analysis of Symmetric DiMethyl-Histone H4-R3 in paraffin-embedded rat colon tissue using Symmetric DiMethyl-Histone H4-R3 Rabbit polyclonal antibody (STJ23979) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
Immunohistochemistry analysis of Symmetric DiMethyl-Histone H4-R3 in paraffin-embedded mouse intestin tissue using Symmetric DiMethyl-Histone H4-R3 Rabbit polyclonal antibody (STJ23979) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
Dot-blot analysis of all sorts of methylation peptides using Symmetric DiMethyl-Histone H4-R3 antibody (STJ23979).
Western blot analysis of various lysates, using Symmetric DiMethyl-Histone H4-R3 Rabbit polyclonal antibody (STJ23979) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Western blot analysis of various lysates, using Symmetric DiMethyl-Histone H4-R3 Rabbit polyclonal antibody (STJ23979) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Di-Methyl-Histone H4-R3 antibody (2-19) (STJ23979)

SKU:

STJ23979

£129.50 - £639.50

Shipping:

Free Shipping

Current Stock:

General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Rabbit
Applications:	WB/IHC/IF
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Di-Methyl-Symmetric Histone H4-R3 (2-19) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	DB 1:500-1:2000 WB 1:500-1:2000 IHC-P 1:50-1:200 IF/ICC 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	H4C1.H4C2.H4C3.H4C4.H4C5.H4C6.H4C8.H4C9.H4C11.H4C12.H4C13.H4C14.H4C15.H4C16
Gene ID:	121504/554313/8294/8359/8360/8361/8362/8363/8364/8365/8366/8367/8368/8370
Uniprot ID:	H4_HUMAN
Immunogen Region:	2-19
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 2-19 of human Histone H4 (NP_003529.1).
Immunogen Sequence:	KGGAKRHRK[ME2]VLRD

Post Translational Modifications	Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin. Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation. Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage. Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by KMT5A/SET8. Dimethylation and trimethylation is performed by KMT5B and KMT5C and induces gene silencing. Monomethylated at Lys-13 (H4K12me1) by N6AMT1.H4K12me1 modification is present at the promoters of numerous genes encoding cell cycle regulators. Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4. Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me). Ufmylated.monofmylated by UFL1 at Lys-32 (H4K31Ufm1) in response to DNA damage. Sumoylated, which is associated with transcriptional repression. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. Butyrylation of histones marks active promoters and competes with histone acetylation. Glutarylation at Lys-92 (H4K91glu) destabilizes nucleosomes by promoting dissociation of the H2A-H2B dimers from nucleosomes. Lactylated in macrophages by EP300/P300 by using lactoyl-CoA directly derived from endogenous or exogenous lactate, leading to stimulates gene transcription.
Function	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Protein Name	Histone H4
Database Links	Reactome: R-HSA-110328 Reactome: R-HSA-110329 Reactome: R-HSA-110330 Reactome: R-HSA-110331 Reactome: R-HSA-1221632 Reactome: R-HSA-171306 Reactome: R-HSA-1912408 Reactome: R-HSA-201722 Reactome: R-HSA-212300 Reactome: R-HSA-2299718 Reactome: R-HSA-2559580 Reactome: R-HSA-2559582 Reactome: R-HSA-2559586 Reactome: R-HSA-3214815 Reactome: R-HSA-3214841 Reactome: R-HSA-3214842 Reactome: R-HSA-3214847 Reactome: R-HSA-3214858 Reactome: R-HSA-427359 Reactome: R-HSA-427389 Reactome: R-HSA-427413 Reactome: R-HSA-4551638 Reactome: R-HSA-5250924 Reactome: R-HSA-5334118 Reactome: R-HSA-5578749 Reactome: R-HSA-5617472 Reactome: R-HSA-5625886 Reactome: R-HSA-5693565 Reactome: R-HSA-5693571 Reactome: R-HSA-5693607 Reactome: R-HSA-606279 Reactome: R-HSA-68616 Reactome: R-HSA-69473 Reactome: R-HSA-73728 Reactome: R-HSA-73772 Reactome: R-HSA-8936459 Reactome: R-HSA-8939236 Reactome: R-HSA-9018519 Reactome: R-HSA-912446 Reactome: R-HSA-9609690 Reactome: R-HSA-9610379 Reactome: R-HSA-9616222 Reactome: R-HSA-9670095 Reactome: R-HSA-9710421 Reactome: R-HSA-977225
Cellular Localisation	Nucleus Chromosome
Alternative Antibody Names	Anti-Histone H4 antibody Anti-H4C1 antibody Anti-H4 antibody Anti-A antibody Anti-H4FA antibody Anti-HIST1H4A.H4C2 antibody Anti-H4 antibody Anti-I antibody Anti-H4FI antibody Anti-HIST1H4B.H4C3 antibody Anti-H4 antibody Anti-G antibody Anti-H4FG antibody Anti-HIST1H4C.H4C4 antibody Anti-H4 antibody Anti-B antibody Anti-H4FB antibody Anti-HIST1H4D.H4C5 antibody Anti-H4 antibody Anti-J antibody Anti-H4FJ antibody Anti-HIST1H4E.H4C6 antibody Anti-H4 antibody Anti-C antibody Anti-H4FC antibody Anti-HIST1H4F.H4C8 antibody Anti-H4 antibody Anti-H antibody Anti-H4FH antibody Anti-HIST1H4H.H4C9 antibody Anti-H4 antibody Anti-M antibody Anti-H4FM antibody Anti-HIST1H4I.H4C11 antibody Anti-H4 antibody Anti-E antibody Anti-H4FE antibody Anti-HIST1H4J.H4C12 antibody Anti-H4 antibody Anti-D antibody Anti-H4FD antibody Anti-HIST1H4K.H4C13 antibody Anti-H4 antibody Anti-K antibody Anti-H4FK antibody Anti-HIST1H4L.H4C14 antibody Anti-H4 antibody Anti-N antibody Anti-H4F2 antibody Anti-H4FN antibody Anti-HIST2H4 antibody Anti-HIST2H4A.H4C15 antibody Anti-H4 antibody Anti-O antibody Anti-H4FO antibody Anti-HIST2H4B.H4C16 antibody Anti-H4-16 antibody Anti-HIST4H4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

reviews