• Western blot analysis of extracts of various cell lines, using DHX30 antibody (STJ111310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using DHX30 antibody (STJ111310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-DHX30 antibody (906-1155) (STJ111310)

SKU:
STJ111310

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DHX30 (906-1155) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DHX30
Gene ID: 22907
Uniprot ID: DHX30_HUMAN
Immunogen Region: 906-1155
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 906-1155 of human DHX30 (NP_055781.2).
Immunogen Sequence: GWEEVLRWQDRSSRENYLEE NLLYAPSLRFIHGLIKQFSE NIYEAFLVGKPSDCTLASAQ CNEYSEEEELVKGVLMAGLY PNLIQVRQGKVTRQGKFKPN SVTYRTKSGNILLHKSTINR EATRLRSRWLTYFMAVKSNG SVFVRDSSQVHPLAVLLLTD GDVHIRDDGRRATISLSDSD LLRLEGDSRTVRLLKELRRA LGRMVERSLRSELAALPPSV QEEHGQLLALLAELLRGPC
Post Translational Modifications Isoform 2: Phosphorylated on Ser-15.
Function RNA-dependent helicase. Plays an important role in the assembly of the mitochondrial large ribosomal subunit. Required for optimal function of the zinc-finger antiviral protein ZC3HAV1. Associates with mitochondrial DNA. Involved in nervous system development and differentiation through its involvement in the up-regulation of a number of genes which are required for neurogenesis, including GSC, NCAM1, neurogenin, and NEUROD.
Protein Name Atp-Dependent Rna Helicase Dhx30
Deah Box Protein 30
Cellular Localisation Cytoplasm
Mitochondrion
Mitochondrion Matrix
Mitochondrion Nucleoid
Localizes To Mitochondrial Rna Granules Found In Close Proximity To The Mitochondrial Nucleoids
Relocalizes To Stress Granules Upon Heat Stress
Alternative Antibody Names Anti-Atp-Dependent Rna Helicase Dhx30 antibody
Anti-Deah Box Protein 30 antibody
Anti-DHX30 antibody
Anti-DDX30 antibody
Anti-KIAA0890 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance