• Immunofluorescence analysis of A-549 cells using DHFR antibody (STJ23375).
  • Western blot analysis of extracts of various cell lines, using DHFR antibody (STJ23375) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-DHFR antibody (1-187) (STJ23375)

SKU:
STJ23375

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DHFR (1-187) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DHFR
Gene ID: 1719
Uniprot ID: DYR_HUMAN
Immunogen Region: 1-187
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-187 of human DHFR (NP_000782.1).
Immunogen Sequence: MVGSLNCIVAVSQNMGIGKN GDLPWPPLRNEFRYFQRMTT TSSVEGKQNLVIMGKKTWFS IPEKNRPLKGRINLVLSREL KEPPQGAHFLSRSLDDALKL TEQPELANKVDMVWIVGGSS VYKEAMNHPGHLKLFVTRIM QDFESDTFFPEIDLEKYKLL PEYPGVLSDVQEEKGIKYKF EVYEKND
Tissue Specificity Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood. Expression is higher in the adult brain than in the fetal brain.
Function Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.
Protein Name Dihydrofolate Reductase
Database Links Reactome: R-HSA-1474151
Reactome: R-HSA-196757
Reactome: R-HSA-69205
Cellular Localisation Mitochondrion
Cytoplasm
Alternative Antibody Names Anti-Dihydrofolate Reductase antibody
Anti-DHFR antibody

Information sourced from Uniprot.org

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