• Western blot analysis of extracts of various cell lines, using DGKA Rabbit polyclonal antibody antibody (STJ28976) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-DGKA antibody (1-200) (STJ28976)

SKU:
STJ28976

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DGKA (1-200) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DGKA
Gene ID: 1606
Uniprot ID: DGKA_HUMAN
Immunogen Region: 1-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human DGKA (NP_958852.1).
Immunogen Sequence: MAKERGLISPSDFAQLQKYM EYSTKKVSDVLKLFEDGEMA KYVQGDAIGYEGFQQFLKIY LEVDNVPRHLSLALFQSFET GHCLNETNVTKDVVCLNDVS CYFSLLEGGRPEDKLEFTFK LYDTDRNGILDSSEVDKIIL QMMRVAEYLDWDVSELRPIL QEMMKEIDYDGSGSVSQAEW VRAGATTVPLLVLLGLEMTL
Tissue Specificity Expressed in lymphocytes.
Function Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids (Probable). Can also phosphorylate 1-alkyl-2-acylglycerol in vitro as efficiently as diacylglycerol provided it contains an arachidonoyl group. Also involved in the production of alkyl-lysophosphatidic acid, another bioactive lipid, through the phosphorylation of 1-alkyl-2-acetyl glycerol.
Protein Name Diacylglycerol Kinase Alpha
Dag Kinase Alpha
80 Kda Diacylglycerol Kinase
Diglyceride Kinase Alpha
Dgk-Alpha
Database Links Reactome: R-HSA-114508
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Diacylglycerol Kinase Alpha antibody
Anti-Dag Kinase Alpha antibody
Anti-80 Kda Diacylglycerol Kinase antibody
Anti-Diglyceride Kinase Alpha antibody
Anti-Dgk-Alpha antibody
Anti-DGKA antibody
Anti-DAGK antibody
Anti-DAGK1 antibody

Information sourced from Uniprot.org

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