| Host: | Goat |
| Applications: | Pep-ELISA/IHC |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-Desmoplakin (1672-1684) is suitable for use in ELISA and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:4000.WB: Preliminary experiments in lysates of cell lines HEK293, HeLa and NIH3T3 gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any fee |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | DSP |
| Gene ID: | 1832 |
| Uniprot ID: | DESP_HUMAN |
| Immunogen Region: | 1672-1684 |
| Accession Number: | NP_004406.2 |
| Specificity: | This antibody is expected to recognize reported isoform I (NP_004406.2) only. |
| Immunogen Sequence: | QESVKQAHLRNEH |
| Function | Major high molecular weight protein of desmosomes. Regulates profibrotic gene expression in cardiomyocytes via activation of the MAPK14/p38 MAPK signaling cascade and increase in TGFB1 protein abundance. |
| Protein Name | DesmoplakinDp250/210 Kda Paraneoplastic Pemphigus Antigen |
| Database Links | Reactome: R-HSA-351906Reactome: R-HSA-6798695Reactome: R-HSA-6805567Reactome: R-HSA-6809371Reactome: R-HSA-9696264Reactome: R-HSA-9696273 |
| Cellular Localisation | Cell JunctionDesmosomeCell MembraneCytoplasmLocalizes To Desmosome Precursor Particles In The CytoplasmLocalizes To The Cytoplasm In Undifferentiated Keratinocytes However Becomes Localizes To Both Lateral And Tricellular Cell-Cell Contacts As Differentiation Progresses And As Epithelial Sheet Formation Completes |
| Alternative Antibody Names | Anti-Desmoplakin antibodyAnti-Dp antibodyAnti-250/210 Kda Paraneoplastic Pemphigus Antigen antibodyAnti-DSP antibody |
Information sourced from Uniprot.org
