• Immunohistochemical analysis of paraffin-embedded Mouse Heart Tissue using DesminMouse mAb diluted at 1:200.
  • Immunohistochemical analysis of paraffin-embedded Mouse Skeletal Muscle Tissue using Desmin (mAb diluted at 1:200.
  • Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1, Desmin monoclonal antibody (1B12) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1, Desmin monoclonal antibody (1B12) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1, Desmin monoclonal antibody (1B12) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunofluorescence analysis of human-uterus tissue. 1, Desmin monoclonal antibody (1B12) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-DES antibody [1B12] (STJ97531)

SKU:
STJ97531

£46.00 - £226.00
Current Stock:
Host: Mouse
Applications: IF/IHC
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Desmin is suitable for use in Immunofluorescence and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: 1B12
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IF 1:50-200
IHC 1:100-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DES
Gene ID: 1674
Uniprot ID: DESM_HUMAN
Specificity: Desmin protein detects endogenous levels of Desmin
Immunogen: Recombinant Protein of Desmin
Function Muscle-specific type III intermediate filament essential for proper muscular structure and function. Plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Contributes to the transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells during a short period of cardiomyogenesis and in cardiac side population stem cells in the adult. Plays a role in maintaining an optimal conformation of nebulette (NEB) on heart muscle sarcomeres to bind and recruit cardiac alpha-actin.
Protein Name Desmin
Database Links Reactome: R-HSA-390522
Cellular Localisation Cytoplasm
Myofibril
Sarcomere
Z Line
Cell Membrane
Sarcolemma
Nucleus
Localizes In The Intercalated Disks Which Occur At The Z Line Of Cardiomyocytes
Localizes In The Nucleus Exclusively In Differentiating Cardiac Progenitor Cells And Premature Cardiomyocytes
Alternative Antibody Names Anti-Desmin antibody
Anti-DES antibody

Information sourced from Uniprot.org

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