Anti-DENR antibody (1-198) (STJ115654)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal DENR (1-198) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DENR |
| Gene ID | 8562 |
| Uniprot ID | DENR_HUMAN |
| Immunogen Region | 1-198 |
| Immunogen Sequence | MAADISESSGADCKGDPRNS AKLDADYPLRVLYCGVCSLP TEYCEYMPDVAKCRQWLEKN FPNEFAKLTVENSPKQEAGI SEGQGTAGEEEEKKKQKRGG RGQIKQKKKTVPQKVTIAKI PRAKKKYVTRVCGLATFEID LKEAQRFFAQKFSCGASVTG EDEIIIQGDFTDDIIDVIQE KWPEVDDDSIEDLGEVKK |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human DENR (NP_003668.2). |
Additional Info
| Tissue Specificity | Highly expressed in heart and skeletal muscle and moderately expressed in the brain, placenta, liver and pancreas. Weakly expressed in the lung and kidney. |
| Function | Translation regulator forming a complex with MCTS1 to promote translation reinitiation. Translation reinitiation is the process where the small ribosomal subunit remains attached to the mRNA following termination of translation of a regulatory upstream ORF (uORF), and resume scanning on the same mRNA molecule to initiate translation of a downstream ORF, usually the main ORF (mORF). The MCTS1/DENR complex is pivotal to two linked mechanisms essential for translation reinitiation. Firstly, the dissociation of deacylated tRNAs from post-termination 40S ribosomal complexes during ribosome recycling. Secondly, the recruitment in an EIF2-independent manner of aminoacylated initiator tRNA to P site of 40S ribosomes for a new round of translation. This regulatory mechanism governs the translation of more than 150 genes which translation reinitiation is MCTS1/DENR complex-dependent. |
| Protein Name | Density-Regulated ProteinDrpProtein Drp1Smooth Muscle Cell-Associated Protein 3Smap-3 |
| Database Links | |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Density-Regulated Protein antibodyAnti-Drp antibodyAnti-Protein Drp1 antibodyAnti-Smooth Muscle Cell-Associated Protein 3 antibodyAnti-Smap-3 antibodyAnti-DENR antibodyAnti-DRP1 antibodyAnti-H14 antibody |
Information sourced from Uniprot.org