| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Gene Symbol: |
DENND1A |
| Gene ID: |
57706 |
| Uniprot ID: |
DEN1A_HUMAN |
| Immunogen Region: |
270-527 aa |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 270-527 of human DENND1A (NP_079096.2). |
| Immunogen Sequence: |
VDTNTLETPFDDLQSLPNDV ISSLKNRLKKVSTTTGDGVA RAFLKAQAAFFGSYRNALKI EPEEPITFCEEAFVSHYRSG AMRQFLQNATQLQLFKQFID GRLDLLNSGEGFSDVFEEEI NMGEYAGSDKLYHQWLSTVR KGSGAILNTVKTKANPAMKT VYKFAKDHAKMGIKEVKNRL KQKDIAENGCAPTPEEQLPK TAPSPLVEAKDPKLREDRRP ITVHFGQVRPPRPHVVKRP |
| Post Translational Modifications | Phosphorylated on serine and/or threonine in an Akt-dependent manner. Phosphorylation probably regulates the guanine nucleotide exchange factor (GEF) activity, possibly by disrupting an intramolecular interaction between the DENN domain and the C-terminus of the protein, thereby relieving the autoinhibition. |
| Function | Guanine nucleotide exchange factor (GEF) regulating clathrin-mediated endocytosis through RAB35 activation. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB35 into its active GTP-bound form. Regulates clathrin-mediated endocytosis of synaptic vesicles and mediates exit from early endosomes. Binds phosphatidylinositol-phosphates (PtdInsPs), with some preference for PtdIns(3)P. |
| Protein Name | Denn Domain-Containing Protein 1aConnecdenn 1ConnecdennProtein Fam31a |
| Database Links | Reactome: R-HSA-8876198 |
| Cellular Localisation | Cytoplasmic VesicleClathrin-Coated Vesicle MembranePeripheral Membrane ProteinPresynaptic Cell MembraneAssociates To Membranes Via Lipid-Binding Activity |
| Alternative Antibody Names | Anti-Denn Domain-Containing Protein 1a antibodyAnti-Connecdenn 1 antibodyAnti-Connecdenn antibodyAnti-Protein Fam31a antibodyAnti-DENND1A antibodyAnti-FAM31A antibodyAnti-KIAA1608 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
