• Biotinylated STJ72512 (0. 3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-Delta-Sarcoglycan, Biotinylated antibody (245-257) {Biotin} (STJ73241)

SKU:
STJ73241-100

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Host: Goat
Applications: Pep-ELISA/WB/IHC
Reactivity: Human/Mouse/Rat/Dog/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Delta-Sarcoglycan, Biotinylated (245-257) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Biotin
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.3-1µg/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: SGCD
Gene ID: 6444
Uniprot ID: SGCD_HUMAN
Immunogen Region: 245-257
Accession Number: NP_000328.2; NP_001121681.1
Specificity: This antibody is expected to recognize isoform 1 (NP_000328.2) and isoform 3 (NP_001121681.1).
Immunogen Sequence: RLPHGSYTPTGTR.
Post Translational Modifications Glycosylated. Disulfide bonds are present.
Function Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
Protein Name Delta-Sarcoglycan
Delta-Sg
35 Kda Dystrophin-Associated Glycoprotein
35dag
Cellular Localisation Cell Membrane
Sarcolemma
Single-Pass Type Ii Membrane Protein
Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Delta-Sarcoglycan antibody
Anti-Delta-Sg antibody
Anti-35 Kda Dystrophin-Associated Glycoprotein antibody
Anti-35dag antibody
Anti-SGCD antibody

Information sourced from Uniprot.org

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