• Immunofluorescence analysis of C6 cells using DEK Rabbit monoclonal antibody (STJ11102678) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using DEK Rabbit monoclonal antibody (STJ11102678) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using DEK Rabbit monoclonal antibody (STJ11102678) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using DEK antibody (STJ11102678) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-DEK antibody (51-200) [S8MR] (STJ11102678)

SKU:
STJ11102678

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-DEK (51-200) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DEK
Gene ID: 7913
Uniprot ID: DEK_HUMAN
Immunogen Region: 51-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 51-200 of human DEK (P35659).
Immunogen Sequence: SLIVEGKREKKKVERLTMQV SSLQREPFTIAQGKGQKLCE IERIHFFLSKKKTDELRNLH KLLYNRPGTVSSLKKNVGQF SGFPFEKGSVQYKKKEEMLK KFRNAMLKSICEVLDLERSG VNSELVKRILNFLMHPKPSG KPLPKSKKTC
Tissue Specificity Ubiquitous. Expressed at relatively high levels.
Post Translational Modifications Phosphorylated by CK2. Phosphorylation fluctuates during the cell cycle with a moderate peak during G(1) phase, and weakens the binding of DEK to DNA.
Function Involved in chromatin organization.
Protein Name Protein Dek
Database Links Reactome: R-HSA-5250924
Reactome: R-HSA-8864260
Reactome: R-HSA-9616222
Cellular Localisation Nucleus
Enriched In Regions Where Chromatin Is Decondensed Or Sparse In The Interphase Nuclei
Alternative Antibody Names Anti-Protein Dek antibody
Anti-DEK antibody

Information sourced from Uniprot.org

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