• Western blot analysis of extracts of various cell lines, using DDX50 antibody (STJ113576) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of U-2 OS cells using DDX50 Rabbit polyclonal antibody (STJ113576) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded mouse testis using DDX50 antibody (STJ113576) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of NIH-3T3 cells using DDX50 Rabbit polyclonal antibody (STJ113576) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded mouse brain using DDX50 antibody (STJ113576) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of C6 cells using DDX50 Rabbit polyclonal antibody (STJ113576) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using DDX50 antibody (STJ113576) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of DDX50 in paraffin-embedded mouse brain using DDX50 Rabbit polyclonal antibody (STJ113576) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH-3T3 cells using DDX50 antibody (STJ113576) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of DDX50 in paraffin-embedded mouse testis using DDX50 Rabbit polyclonal antibody (STJ113576) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of U-2 OS cells using DDX50 antibody (STJ113576) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using DDX50 Rabbit polyclonal antibody (STJ113576) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-DDX50 antibody (1-135) (STJ113576)

SKU:
STJ113576

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:200-1:2000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DDX50
Gene ID: 79009
Uniprot ID: DDX50_HUMAN
Immunogen Region: 1-135
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human DDX50 (NP_076950.1).
Immunogen Sequence: MPGKLLWGDIMELEAPLEES ESQKKERQKSDRRKSRHHYD SDEKSETRENGVTDDLDAPK AKKSKMKEKLNGDTEEGFNR LSDEFSKSHKSRRKDLPNGD IDEYEKKSKRVSSLDTSTHK SSDNKLEETLTREQK
Protein Name Atp-Dependent Rna Helicase Ddx50
Dead Box Protein 50
Gu-Beta
Nucleolar Protein Gu2
Cellular Localisation Nucleus
Nucleolus
Alternative Antibody Names Anti-Atp-Dependent Rna Helicase Ddx50 antibody
Anti-Dead Box Protein 50 antibody
Anti-Gu-Beta antibody
Anti-Nucleolar Protein Gu2 antibody
Anti-DDX50 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance