• Western blot analysis of extracts of mouse testis, using DDX4 antibody (STJ118072) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded mouse testis using DDX4 rabbit polyclonal antibody (STJ118072) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of rat testis using DDX4 rabbit polyclonal antibody (STJ118072) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse testis using DDX4 rabbit polyclonal antibody (STJ118072) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat testis cells using DDX4 rabbit polyclonal antibody (STJ118072) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse testis cells using DDX4 rabbit polyclonal antibody (STJ118072) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-DDX4 antibody (635-724) (STJ118072)

SKU:
STJ118072

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DDX4
Gene ID: 54514
Uniprot ID: DDX4_HUMAN
Immunogen Region: 635-724
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 635-724 of human DDX4 (NP_077726.1).
Immunogen Sequence: GNTGRAISFFDLESDNHLAQ PLVKVLTDAQQDVPAWLEEI AFSTYIPGFSGSTRGNVFAS VDTRKGKSTLNTAGFSSSQA PNPVDDESWD
Tissue Specificity Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes.
Function ATP-dependent RNA helicase required during spermatogenesis. Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle. Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs.
Protein Name Probable Atp-Dependent Rna Helicase Ddx4
Dead Box Protein 4
Vasa Homolog
Database Links Reactome: R-HSA-5601884
Reactome: R-HSA-9696264
Cellular Localisation Cytoplasm
Perinuclear Region
Component Of The Meiotic Nuage
Also Named P Granule
A Germ-Cell-Specific Organelle Required To Repress Transposon Activity During Meiosis
Alternative Antibody Names Anti-Probable Atp-Dependent Rna Helicase Ddx4 antibody
Anti-Dead Box Protein 4 antibody
Anti-Vasa Homolog antibody
Anti-DDX4 antibody
Anti-VASA antibody

Information sourced from Uniprot.org

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