• Western blot analysis of Mouse Liver Tissue Lysate using CHOP Mouse mAb diluted at 1:2000.
  • Immunohistochemical analysis of paraffin-embedded Human Pancreas Carcinoma Tissue using CHOP Mouse mAb diluted at 1:200.
  • Immunohistochemical analysis of paraffin-embedded Human Stomach Carcinoma Tissue using CHOP Mouse mAb diluted at 1:200.
  • Immunofluorescence analysis of Mouse-brain tissue. 1, CHOP Mouse monoclonal antibody (2B1) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1, CHOP Mouse monoclonal antibody (2B1) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunofluorescence analysis of Hela cell. 1, Calnexin Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-DDIT3 antibody (10-90 aa) [2B1] (STJ97774)

SKU:
STJ97774

£46.00 - £226.00
Current Stock:
Host: Mouse
Applications: WB/IF/IHC
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-DNA damage-inducible transcript 3 protein (10-90 aa) is suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: 2B1
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:1000-2000
IHC 1:100-200
IF 1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DDIT3
Gene ID: 1649
Uniprot ID: DDIT3_HUMAN
Immunogen Region: 10-90 aa
Specificity: CHOP protein detects endogenous levels of DDIT3
Immunogen: Synthetic peptide of CHOP at the amino acid range of 10-90
Function Multifunctional transcription factor in endoplasmic reticulum (ER) stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L. Negatively regulates.expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG). Together with ATF4, mediates ER-mediated cell death by promoting expression of genes involved in cellular amino acid metabolic processes, mRNA translation and the unfolded protein response (UPR) in response to ER stress. Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding properties and repressing its transcriptional activity. Plays a regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response. Acts as a major regulator of postnatal neovascularization through regulation of endothelial nitric oxide synthase (NOS3)-related signaling.
Protein Name Dna Damage-Inducible Transcript 3 Protein
Ddit-3
C/Ebp Zeta
C/Ebp-Homologous Protein
Chop
C/Ebp-Homologous Protein 10
Chop-10
Ccaat/Enhancer-Binding Protein Homologous Protein
Growth Arrest And Dna Damage-Inducible Protein Gadd153
Database Links Reactome: R-HSA-380994
Reactome: R-HSA-381183
Reactome: R-HSA-9614657
Reactome: R-HSA-9633012
Reactome: R-HSA-9648895
Cellular Localisation Cytoplasm
Nucleus
Present In The Cytoplasm Under Non-Stressed Conditions And Er Stress Leads To Its Nuclear Accumulation
Alternative Antibody Names Anti-Dna Damage-Inducible Transcript 3 Protein antibody
Anti-Ddit-3 antibody
Anti-C/Ebp Zeta antibody
Anti-C/Ebp-Homologous Protein antibody
Anti-Chop antibody
Anti-C/Ebp-Homologous Protein 10 antibody
Anti-Chop-10 antibody
Anti-Ccaat/Enhancer-Binding Protein Homologous Protein antibody
Anti-Growth Arrest And Dna Damage-Inducible Protein Gadd153 antibody
Anti-DDIT3 antibody
Anti-CHOP antibody
Anti-CHOP10 antibody
Anti-GADD153 antibody

Information sourced from Uniprot.org

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