Western blot analysis of 293T, using mouse anti DDDDK-Tag monoclonal antibody antibody (STJ11103697) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-mouse IgG (H+L) (STJS000854) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-DDDDK-Tag antibody [S7MM] (STJ11103697)

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STJ11103697
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Covered by St John's Laboratory Guarantee

Host: Mouse
Applications: WB/IF/ICC/IP/FC/ELISA
Reactivity: Species independent
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Monoclonal
Clone ID : S7MM
Conjugation: Unconjugated
Isotype: IgG1k
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:20000-1:80000
IF/ICC:1:50-1:200
IP:0.5 Mu g-4 Mu g antibody for 100 Mu g-200 Mu g extracts of whole cells
FC (intra) :5 Mu l per 10^6 cells in 100 Mu l volume
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Specificity: A synthetic peptide corresponding to DDDDK tag.
Immunogen Sequence: DYKDDDDK
Background FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.

Information sourced from Uniprot.org

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